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1tez

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[[Image:1tez.gif|left|200px]]<br /><applet load="1tez" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1tez.gif|left|200px]]
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caption="1tez, resolution 1.80&Aring;" />
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'''COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS'''<br />
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{{Structure
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|PDB= 1tez |SIZE=350|CAPTION= <scene name='initialview01'>1tez</scene>, resolution 1.80&Aring;
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|SITE= <scene name='pdbsite=ACT:Upon+Complexation+w.+The+Cpd-Damage+The+Active+Site+Appa+...'>ACT</scene>
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=HDF:8-HYDROXY-10-(D-RIBO-2,3,4,5-TETRAHYDROXYPENTYL)-5-DEAZAISOALLOXAZINE'>HDF</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Deoxyribodipyrimidine_photo-lyase Deoxyribodipyrimidine photo-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.3 4.1.99.3]
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|GENE= PHR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1131 Synechococcus sp.])
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}}
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'''COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1TEZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=HDF:'>HDF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deoxyribodipyrimidine_photo-lyase Deoxyribodipyrimidine photo-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.99.3 4.1.99.3] Known structural/functional Site: <scene name='pdbsite=ACT:Upon+Complexation+w.+The+Cpd-Damage+The+Active+Site+Appa+...'>ACT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEZ OCA].
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1TEZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEZ OCA].
==Reference==
==Reference==
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Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15576622 15576622]
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Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15576622 15576622]
[[Category: Deoxyribodipyrimidine photo-lyase]]
[[Category: Deoxyribodipyrimidine photo-lyase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: photolyase; dna repair; light-driven electron transfer]]
[[Category: photolyase; dna repair; light-driven electron transfer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:12:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:17:28 2008''

Revision as of 12:17, 20 March 2008

Image:1tez.gif


PDB ID 1tez

Drag the structure with the mouse to rotate
, resolution 1.80Å
Sites:
Ligands: , and
Gene: PHR (Synechococcus sp.)
Activity: Deoxyribodipyrimidine photo-lyase, with EC number 4.1.99.3
Coordinates: save as pdb, mmCIF, xml



COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS


Overview

DNA photolyases use light energy to repair DNA that comprises ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine dimers (CPDs). Here we report the crystal structure of a DNA photolyase bound to duplex DNA that is bent by 50 degrees and comprises a synthetic CPD lesion. This CPD lesion is flipped into the active site and split there into two thymines by synchrotron radiation at 100 K. Although photolyases catalyze blue light-driven CPD cleavage only above 200 K, this structure apparently mimics a structural substate during light-driven DNA repair in which back-flipping of the thymines into duplex DNA has not yet taken place.

About this Structure

1TEZ is a Single protein structure of sequence from Synechococcus sp.. Full crystallographic information is available from OCA.

Reference

Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:15576622

Page seeded by OCA on Thu Mar 20 14:17:28 2008

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