1uvi
From Proteopedia
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| - | [[ | + | ==THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 WITH 6NT RNA== |
| + | <StructureSection load='1uvi' size='340' side='right' caption='[[1uvi]], [[Resolution|resolution]] 2.15Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1uvi]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UVI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UVI FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1hhs|1hhs]], [[1hht|1hht]], [[1hi0|1hi0]], [[1hi1|1hi1]], [[1hi8|1hi8]], [[1uvj|1uvj]], [[1uvk|1uvk]], [[1uvl|1uvl]], [[1uvm|1uvm]], [[1uvn|1uvn]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uvi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uvi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uvi RCSB], [http://www.ebi.ac.uk/pdbsum/1uvi PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. We have cocrystallized RNA oligonucleotides with the polymerase, revealing the mode of binding of RNA templates. This binding is somewhat different from that previously seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase complex by the addition of substrate and Mg2+ initiates a single round of reaction within the crystal to form a dead-end complex that partially collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we have been able to capture the inhibited complex which shows distortion that explains the structural basis for the inhibition of such polymerases by Ca2+. | ||
| - | + | The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase.,Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM Structure. 2004 Feb;12(2):307-16. PMID:14962391<ref>PMID:14962391</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[RNA polymerase|RNA polymerase]] | *[[RNA polymerase|RNA polymerase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| - | [[Category: Bamford, D | + | </StructureSection> |
| - | [[Category: Butcher, S | + | [[Category: Bamford, D]] |
| - | [[Category: Grimes, J M | + | [[Category: Butcher, S]] |
| - | [[Category: Makeyev, E V | + | [[Category: Grimes, J M]] |
| - | [[Category: Salgado, P S | + | [[Category: Makeyev, E V]] |
| - | [[Category: Stuart, D I | + | [[Category: Salgado, P S]] |
| + | [[Category: Stuart, D I]] | ||
[[Category: Polymerase]] | [[Category: Polymerase]] | ||
[[Category: Polymerase-complex]] | [[Category: Polymerase-complex]] | ||
Revision as of 06:43, 22 December 2014
THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 WITH 6NT RNA
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