1tj7

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[[Image:1tj7.gif|left|200px]]<br /><applet load="1tj7" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1tj7.gif|left|200px]]
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caption="1tj7, resolution 2.44&Aring;" />
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'''Structure determination and refinement at 2.44 A resolution of Argininosuccinate lyase from E. coli'''<br />
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{{Structure
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|PDB= 1tj7 |SIZE=350|CAPTION= <scene name='initialview01'>1tj7</scene>, resolution 2.44&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1]
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|GENE=
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}}
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'''Structure determination and refinement at 2.44 A resolution of Argininosuccinate lyase from E. coli'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1TJ7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJ7 OCA].
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1TJ7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJ7 OCA].
==Reference==
==Reference==
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Structure determination and refinement at 2.44 A resolution of argininosuccinate lyase from Escherichia coli., Bhaumik P, Koski MK, Bergmann U, Wierenga RK, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):1964-70. Epub 2004, Oct 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15502303 15502303]
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Structure determination and refinement at 2.44 A resolution of argininosuccinate lyase from Escherichia coli., Bhaumik P, Koski MK, Bergmann U, Wierenga RK, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):1964-70. Epub 2004, Oct 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15502303 15502303]
[[Category: Argininosuccinate lyase]]
[[Category: Argininosuccinate lyase]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: fumarase]]
[[Category: fumarase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:14:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:19:03 2008''

Revision as of 12:19, 20 March 2008

Image:1tj7.gif


PDB ID 1tj7

Drag the structure with the mouse to rotate
, resolution 2.44Å
Ligands: and
Activity: Argininosuccinate lyase, with EC number 4.3.2.1
Coordinates: save as pdb, mmCIF, xml



Structure determination and refinement at 2.44 A resolution of Argininosuccinate lyase from E. coli


Overview

Escherichia coli argininosuccinate lyase has been crystallized from a highly concentrated sample of purified recombinant alpha-methylacyl-CoA racemase, in which it occurred as a minor impurity. The structure has been solved using molecular replacement at 2.44 A resolution. The enzyme is tetrameric, but in this crystal form there is a dimer in the asymmetric unit. The tetramer has four active sites; each active site is constructed from loops of three different subunits. One of these catalytic loops, near residues Ser277 and Ser278, was disordered in previous structures of active lyases, but is very well ordered in this structure in one of the subunits owing to the presence of two phosphate ions in the active-site cavity. The positions of these phosphate ions indicate a plausible mode of binding of the succinate moiety of the substrate in the competent catalytic complex.

About this Structure

1TJ7 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure determination and refinement at 2.44 A resolution of argininosuccinate lyase from Escherichia coli., Bhaumik P, Koski MK, Bergmann U, Wierenga RK, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):1964-70. Epub 2004, Oct 20. PMID:15502303

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