1tps
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1tps.jpg|left|200px]] | + | [[Image:1tps.jpg|left|200px]] |
| - | + | ||
| - | '''ATOMIC STRUCTURE OF THE TRYPSIN-A90720A COMPLEX: A UNIFIED APPROACH TO STRUCTURE AND FUNCTION''' | + | {{Structure |
| + | |PDB= 1tps |SIZE=350|CAPTION= <scene name='initialview01'>1tps</scene>, resolution 1.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=A9A:1-(SULFOGLYCOLOYL-LEUCYL-THREONYL-VALYL-N-METHYLTYROSYL-LEUCYL)-3-(ARGINYLANYLAMINO)-6-HYDROXY-2-PIPERIDONE'>A9A</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''ATOMIC STRUCTURE OF THE TRYPSIN-A90720A COMPLEX: A UNIFIED APPROACH TO STRUCTURE AND FUNCTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1TPS is a [ | + | 1TPS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPS OCA]. |
==Reference== | ==Reference== | ||
| - | Atomic structure of the trypsin-A90720A complex: a unified approach to structure and function., Lee AY, Smitka TA, Bonjouklian R, Clardy J, Chem Biol. 1994 Oct;1(2):113-7. PMID:[http:// | + | Atomic structure of the trypsin-A90720A complex: a unified approach to structure and function., Lee AY, Smitka TA, Bonjouklian R, Clardy J, Chem Biol. 1994 Oct;1(2):113-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9383379 9383379] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 19: | Line 28: | ||
[[Category: hydrolase(serine protease)]] | [[Category: hydrolase(serine protease)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:21:32 2008'' |
Revision as of 12:21, 20 March 2008
| |||||||
| , resolution 1.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ATOMIC STRUCTURE OF THE TRYPSIN-A90720A COMPLEX: A UNIFIED APPROACH TO STRUCTURE AND FUNCTION
Overview
BACKGROUND: A90720A is a potent serine proteinase inhibitor produced by the terrestrial blue-green alga Microchaete loktakensis. Most of its structure has been defined by spectroscopic and degradative studies, but the configurations of several stereochemical centers are unknown, and its mode of inhibition of serine proteinases is not understood. We therefore examined the structure of the compound in a complex with trypsin. RESULTS: We have crystallized the bovine trypsin-A90720A complex and determined its three-dimensional structure at 1.90 A resolution using single crystal X-ray diffraction. The structure of the bound inhibitor is clearly shown in the electron density. The structure defines the absolute stereostructure of A90720A, establishes its bound conformation and illuminates its mode of inhibition. CONCLUSIONS: A90720A interacts with trypsin in a substrate-like manner through an extensive series of hydrogen bonds, hydrophobic interactions and steric complementarity. The compound uses a mixture of peptidal and nonpeptidal elements to imitate the canonical conformation of the exposed binding loop of 'small' proteinase inhibitors.
About this Structure
1TPS is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Atomic structure of the trypsin-A90720A complex: a unified approach to structure and function., Lee AY, Smitka TA, Bonjouklian R, Clardy J, Chem Biol. 1994 Oct;1(2):113-7. PMID:9383379
Page seeded by OCA on Thu Mar 20 14:21:32 2008
