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1tpg

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[[Image:1tpg.gif|left|200px]]<br /><applet load="1tpg" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1tpg.gif|left|200px]]
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caption="1tpg" />
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'''F1-G MODULE PAIR RESIDUES 1-91 (C83S) OF TISSUE-TYPE PLASMINOGEN ACTIVATOR (T-PA) (NMR, 298K, PH2.95, REPRESENTATIVE STRUCTURE)'''<br />
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{{Structure
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|PDB= 1tpg |SIZE=350|CAPTION= <scene name='initialview01'>1tpg</scene>
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|SITE=
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|LIGAND=
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|ACTIVITY= [http://en.wikipedia.org/wiki/T-plasminogen_activator T-plasminogen activator], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.68 3.4.21.68]
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|GENE=
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}}
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'''F1-G MODULE PAIR RESIDUES 1-91 (C83S) OF TISSUE-TYPE PLASMINOGEN ACTIVATOR (T-PA) (NMR, 298K, PH2.95, REPRESENTATIVE STRUCTURE)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1TPG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/T-plasminogen_activator T-plasminogen activator], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.68 3.4.21.68] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPG OCA].
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1TPG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPG OCA].
==Reference==
==Reference==
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The solution structure and backbone dynamics of the fibronectin type I and epidermal growth factor-like pair of modules of tissue-type plasminogen activator., Smith BO, Downing AK, Driscoll PC, Dudgeon TJ, Campbell ID, Structure. 1995 Aug 15;3(8):823-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7582899 7582899]
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The solution structure and backbone dynamics of the fibronectin type I and epidermal growth factor-like pair of modules of tissue-type plasminogen activator., Smith BO, Downing AK, Driscoll PC, Dudgeon TJ, Campbell ID, Structure. 1995 Aug 15;3(8):823-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7582899 7582899]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: plasminogen activation]]
[[Category: plasminogen activation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:15:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:21:33 2008''

Revision as of 12:21, 20 March 2008


PDB ID 1tpg

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Activity: T-plasminogen activator, with EC number 3.4.21.68
Coordinates: save as pdb, mmCIF, xml



F1-G MODULE PAIR RESIDUES 1-91 (C83S) OF TISSUE-TYPE PLASMINOGEN ACTIVATOR (T-PA) (NMR, 298K, PH2.95, REPRESENTATIVE STRUCTURE)


Contents

Overview

BACKGROUND: The thrombolytic serine protease tissue-type plasminogen activator (t-PA) is a classical modular protein consisting of three types of domain in addition to the serine protease domain: F1 (homologous to fibronectin type I); G (epidermal growth factor-like) and kringle. Biochemical data suggest that the F1 and G modules play a major role in the binding of t-PA to fibrin and to receptors on hepatocytes. RESULTS: We have derived the solution structure of the F1 and G pair of modules from t-PA by two- and three-dimensional NMR techniques, in combination with dynamical simulated annealing calculations. We have also obtained information about the molecule's backbone dynamics through measurement of amide 15N relaxation parameters. CONCLUSIONS: Although the F1 and G modules each adopt their expected tertiary structure, the modules interact intimately to bury a hydrophobic core, and the inter-module linker makes up the third strand of the G module's major beta-sheet. The new structural results allow the interpretation of earlier mutational data relevant to fibrin-binding and hepatocyte-receptor binding.

Disease

Known disease associated with this structure: Plasminogen activator deficiency OMIM:[173370]

About this Structure

1TPG is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The solution structure and backbone dynamics of the fibronectin type I and epidermal growth factor-like pair of modules of tissue-type plasminogen activator., Smith BO, Downing AK, Driscoll PC, Dudgeon TJ, Campbell ID, Structure. 1995 Aug 15;3(8):823-33. PMID:7582899

Page seeded by OCA on Thu Mar 20 14:21:33 2008

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