1tsv
From Proteopedia
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| - | [[Image:1tsv.jpg|left|200px]] | + | [[Image:1tsv.jpg|left|200px]] |
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| - | '''THYMIDYLATE SYNTHASE R179A MUTANT''' | + | {{Structure |
| + | |PDB= 1tsv |SIZE=350|CAPTION= <scene name='initialview01'>1tsv</scene>, resolution 2.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=UMP:2'-DEOXYURIDINE 5'-MONOPHOSPHATE'>UMP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THYMIDYLATE SYNTHASE R179A MUTANT''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1TSV is a [ | + | 1TSV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_casei Lactobacillus casei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TSV OCA]. |
==Reference== | ==Reference== | ||
| - | Contribution of a salt bridge to binding affinity and dUMP orientation to catalytic rate: mutation of a substrate-binding arginine in thymidylate synthase., Finer-Moore JS, Fauman EB, Morse RJ, Santi DV, Stroud RM, Protein Eng. 1996 Jan;9(1):69-75. PMID:[http:// | + | Contribution of a salt bridge to binding affinity and dUMP orientation to catalytic rate: mutation of a substrate-binding arginine in thymidylate synthase., Finer-Moore JS, Fauman EB, Morse RJ, Santi DV, Stroud RM, Protein Eng. 1996 Jan;9(1):69-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9053905 9053905] |
[[Category: Lactobacillus casei]] | [[Category: Lactobacillus casei]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:22:40 2008'' |
Revision as of 12:22, 20 March 2008
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| , resolution 2.9Å | |||||||
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| Ligands: | |||||||
| Activity: | Thymidylate synthase, with EC number 2.1.1.45 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THYMIDYLATE SYNTHASE R179A MUTANT
Overview
Invariant arginine 179, one of four arginines that are conserved in all thymidylate synthases (TS) and that bind the phosphate moiety of the substrate 2'-deoxyuridine-5'-monophosphate (dUMP), can be altered even to a negatively charged glutamic acid with little effect on kcat. In the mutant structures, ordered water or the other phosphate-binding arginines compensate for the hydrogen bonds made by Arg179 in the wild-type enzyme and there is almost no change in the conformation or binding site of dUMP. Correlation of dUMP Kds for TS R179A and TS R179K with the structures of their binary complexes shows, that the positive charge on Arg179 contributes significantly to dUMP binding affinity. kcat/K(m) for dUMP measures the rate of dUMP binding to TS during the ordered bi-substrate reaction, and in the ternary complex dUMP provides a binding surface for the cofactor. kcat/K(m) reflects the ability of the enzyme to accept a properly oriented dUMP for catalysis and is less sensitive than is Kd to the changes in electrostatics at the phosphate binding site.
About this Structure
1TSV is a Single protein structure of sequence from Lactobacillus casei. Full crystallographic information is available from OCA.
Reference
Contribution of a salt bridge to binding affinity and dUMP orientation to catalytic rate: mutation of a substrate-binding arginine in thymidylate synthase., Finer-Moore JS, Fauman EB, Morse RJ, Santi DV, Stroud RM, Protein Eng. 1996 Jan;9(1):69-75. PMID:9053905
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