1tuf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1tuf.gif|left|200px]]<br /><applet load="1tuf" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1tuf.gif|left|200px]]
-
caption="1tuf, resolution 2.40&Aring;" />
+
 
-
'''Crystal structure of Diaminopimelate Decarboxylase from m. jannaschi'''<br />
+
{{Structure
 +
|PDB= 1tuf |SIZE=350|CAPTION= <scene name='initialview01'>1tuf</scene>, resolution 2.40&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=AZ1:AZELAIC ACID'>AZ1</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Diaminopimelate_decarboxylase Diaminopimelate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.20 4.1.1.20]
 +
|GENE= LYSA, MJ1097 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])
 +
}}
 +
 
 +
'''Crystal structure of Diaminopimelate Decarboxylase from m. jannaschi'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1TUF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=AZ1:'>AZ1</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Diaminopimelate_decarboxylase Diaminopimelate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.20 4.1.1.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TUF OCA].
+
1TUF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TUF OCA].
==Reference==
==Reference==
-
Cocrystal structures of diaminopimelate decarboxylase: mechanism, evolution, and inhibition of an antibiotic resistance accessory factor., Ray SS, Bonanno JB, Rajashankar KR, Pinho MG, He G, De Lencastre H, Tomasz A, Burley SK, Structure. 2002 Nov;10(11):1499-508. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12429091 12429091]
+
Cocrystal structures of diaminopimelate decarboxylase: mechanism, evolution, and inhibition of an antibiotic resistance accessory factor., Ray SS, Bonanno JB, Rajashankar KR, Pinho MG, He G, De Lencastre H, Tomasz A, Burley SK, Structure. 2002 Nov;10(11):1499-508. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12429091 12429091]
[[Category: Diaminopimelate decarboxylase]]
[[Category: Diaminopimelate decarboxylase]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
Line 31: Line 40:
[[Category: protein structure initiative]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: psi]]
-
[[Category: structural genomics]]
+
[[Category: structural genomic]]
[[Category: t135]]
[[Category: t135]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:17:26 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:23:21 2008''

Revision as of 12:23, 20 March 2008

Image:1tuf.gif


PDB ID 1tuf

Drag the structure with the mouse to rotate
, resolution 2.40Å
Ligands:
Gene: LYSA, MJ1097 (Methanocaldococcus jannaschii)
Activity: Diaminopimelate decarboxylase, with EC number 4.1.1.20
Coordinates: save as pdb, mmCIF, xml



Crystal structure of Diaminopimelate Decarboxylase from m. jannaschi


Overview

Cocrystal structures of Methanococcus jannaschii diaminopimelate decarboxylase (DAPDC) bound to a substrate analog, azelaic acid, and its L-lysine product have been determined at 2.6 A and 2.0 A, respectively. This PLP-dependent enzyme is responsible for the final step of L-lysine biosynthesis in bacteria and plays a role in beta-lactam antibiotic resistance in Staphylococcus aureus. Substrate specificity derives from recognition of the L-chiral center of diaminopimelate and a system of ionic "molecular rulers" that dictate substrate length. A coupled-enzyme assay system permitted measurement of kinetic parameters for recombinant DAPDCs and inhibition constants (K(i)) for azelaic acid (89 microM) and other substrate analogs. Implications for rational design of broad-spectrum antimicrobial agents targeted against DAPDCs of drug-resistant strains of bacterial pathogens, such as Staphylococcus aureus, are discussed.

About this Structure

1TUF is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

Reference

Cocrystal structures of diaminopimelate decarboxylase: mechanism, evolution, and inhibition of an antibiotic resistance accessory factor., Ray SS, Bonanno JB, Rajashankar KR, Pinho MG, He G, De Lencastre H, Tomasz A, Burley SK, Structure. 2002 Nov;10(11):1499-508. PMID:12429091

Page seeded by OCA on Thu Mar 20 14:23:21 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tuf"
Personal tools