1tvm
From Proteopedia
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| - | [[Image:1tvm.gif|left|200px]] | + | [[Image:1tvm.gif|left|200px]] |
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| - | '''NMR structure of enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system''' | + | {{Structure |
| + | |PDB= 1tvm |SIZE=350|CAPTION= <scene name='initialview01'>1tvm</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] | ||
| + | |GENE= GATB, C2618, Z3256, ECS2896, SF2155, S2281 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''NMR structure of enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1TVM is a [ | + | 1TVM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TVM OCA]. |
==Reference== | ==Reference== | ||
| - | NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA., Volpon L, Young CR, Matte A, Gehring K, Protein Sci. 2006 Oct;15(10):2435-41. Epub 2006 Sep 8. PMID:[http:// | + | NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA., Volpon L, Young CR, Matte A, Gehring K, Protein Sci. 2006 Oct;15(10):2435-41. Epub 2006 Sep 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16963640 16963640] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein-N(pi)-phosphohistidine--sugar phosphotransferase]] | [[Category: Protein-N(pi)-phosphohistidine--sugar phosphotransferase]] | ||
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[[Category: bsgi]] | [[Category: bsgi]] | ||
[[Category: e. coli]] | [[Category: e. coli]] | ||
| - | [[Category: four-stranded parallel open twisted beta sheet flanked by alpha helices on both | + | [[Category: four-stranded parallel open twisted beta sheet flanked by alpha helices on both side]] |
[[Category: montreal-kingston bacterial structural genomics initiative]] | [[Category: montreal-kingston bacterial structural genomics initiative]] | ||
[[Category: p-loop]] | [[Category: p-loop]] | ||
[[Category: phosphotransferase system (pts)]] | [[Category: phosphotransferase system (pts)]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:23:48 2008'' |
Revision as of 12:23, 20 March 2008
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| Gene: | GATB, C2618, Z3256, ECS2896, SF2155, S2281 (Escherichia coli) | ||||||
| Activity: | Protein-N(pi)-phosphohistidine--sugar phosphotransferase, with EC number 2.7.1.69 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR structure of enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system
Overview
The phosphoenolpyruvate-dependent carbohydrate transport system (PTS) couples uptake with phosphorylation of a variety of carbohydrates in prokaryotes. In this multienzyme complex, the enzyme II (EII), a carbohydrate-specific permease, is constituted of two cytoplasmic domains, IIA and IIB, and a transmembrane channel IIC domain. Among the five families of EIIs identified in Escherichia coli, the galactitol-specific transporter (II(gat)) belongs to the glucitol family and is structurally the least well-characterized. Here, we used nuclear magnetic resonance (NMR) spectroscopy to solve the three-dimensional structure of the IIB subunit (GatB). GatB consists of a central four-stranded parallel beta-sheet flanked by alpha-helices on both sides; the active site cysteine of GatB is located at the beginning of an unstructured loop between beta1 and alpha1 that folds into a P-loop-like structure. This structural arrangement shows similarities with other IIB subunits but also with mammalian low molecular weight protein tyrosine phosphatases (LMW PTPase) and arsenate reductase (ArsC). An NMR titration was performed to identify the GatA-interacting residues.
About this Structure
1TVM is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
NMR structure of the enzyme GatB of the galactitol-specific phosphoenolpyruvate-dependent phosphotransferase system and its interaction with GatA., Volpon L, Young CR, Matte A, Gehring K, Protein Sci. 2006 Oct;15(10):2435-41. Epub 2006 Sep 8. PMID:16963640
Page seeded by OCA on Thu Mar 20 14:23:48 2008
Categories: Escherichia coli | Protein-N(pi)-phosphohistidine--sugar phosphotransferase | Single protein | BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative. | Cygler, M. | Gehring, K. | Iannuzzi, P. | Lim, N S. | Volpon, L. | Young, C R. | Bsgi | E. coli | Four-stranded parallel open twisted beta sheet flanked by alpha helices on both side | Montreal-kingston bacterial structural genomics initiative | P-loop | Phosphotransferase system (pts) | Structural genomic
