1txd
From Proteopedia
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| - | [[Image:1txd.jpg|left|200px]] | + | [[Image:1txd.jpg|left|200px]] |
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| - | '''Crystal Structure of the DH/PH domains of Leukemia-associated RhoGEF''' | + | {{Structure |
| + | |PDB= 1txd |SIZE=350|CAPTION= <scene name='initialview01'>1txd</scene>, resolution 2.13Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= ARHGEF12, LARG, KIAA0382 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the DH/PH domains of Leukemia-associated RhoGEF''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1TXD is a [ | + | 1TXD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXD OCA]. |
==Reference== | ==Reference== | ||
| - | Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine-nucleotide exchange factor., Kristelly R, Gao G, Tesmer JJ, J Biol Chem. 2004 Nov 5;279(45):47352-62. Epub 2004 Aug 25. PMID:[http:// | + | Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine-nucleotide exchange factor., Kristelly R, Gao G, Tesmer JJ, J Biol Chem. 2004 Nov 5;279(45):47352-62. Epub 2004 Aug 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15331592 15331592] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: helical bundle (dh)]] | [[Category: helical bundle (dh)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:24:26 2008'' |
Revision as of 12:24, 20 March 2008
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| , resolution 2.13Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | ARHGEF12, LARG, KIAA0382 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the DH/PH domains of Leukemia-associated RhoGEF
Contents |
Overview
Rho guanine-nucleotide exchange factors (RhoGEFs) activate Rho GTPases, and thereby regulate cytoskeletal structure, gene transcription, and cell migration. Leukemia-associated RhoGEF (LARG) belongs to a small subfamily of RhoGEFs that are RhoA-selective and directly activated by the Galpha12/13 family of heterotrimeric G proteins. Herein we describe the atomic structures of the catalytic Dbl homology (DH) and pleckstrin homology (PH) domains of LARG alone and in complex with RhoA. These structures demonstrate that the DH/PH domains of LARG can undergo a dramatic conformational change upon binding RhoA, wherein both the DH and PH domains directly engage RhoA. Through mutational analysis we show that full nucleotide exchange activity requires a novel N-terminal extension on the DH domain that is predicted to exist in a broader family of RhoGEFs that includes p115-RhoGEF, Lbc, Lfc, Net1, and Xpln, and identify regions within the LARG PH domain that contribute to its ability to facilitate nucleotide exchange in vitro. In crystals of the DH/PH-RhoA complex, the active site of RhoA adopts two distinct GDP-excluding conformations among the four unique complexes in the asymmetric unit. Similar changes were previously observed in structures of nucleotide-free Ras and Ef-Tu. A potential protein-docking site on the LARG PH domain is also evident and appears to be conserved throughout the Lbc subfamily of RhoGEFs.
Disease
Known diseases associated with this structure: Leukemia, acute myeloid OMIM:[604763]
About this Structure
1TXD is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine-nucleotide exchange factor., Kristelly R, Gao G, Tesmer JJ, J Biol Chem. 2004 Nov 5;279(45):47352-62. Epub 2004 Aug 25. PMID:15331592
Page seeded by OCA on Thu Mar 20 14:24:26 2008
