1tzy

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1tzy.jpg|left|200px]]<br /><applet load="1tzy" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1tzy.jpg|left|200px]]
-
caption="1tzy, resolution 1.90&Aring;" />
+
 
-
'''Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution'''<br />
+
{{Structure
 +
|PDB= 1tzy |SIZE=350|CAPTION= <scene name='initialview01'>1tzy</scene>, resolution 1.90&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
 +
|ACTIVITY=
 +
|GENE=
 +
}}
 +
 
 +
'''Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1TZY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZY OCA].
+
1TZY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZY OCA].
==Reference==
==Reference==
-
High-resolution structure of the native histone octamer., Wood CM, Nicholson JM, Lambert SJ, Chantalat L, Reynolds CD, Baldwin JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jun 1;61(Pt, 6):541-5. Epub 2005 Jun 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511091 16511091]
+
High-resolution structure of the native histone octamer., Wood CM, Nicholson JM, Lambert SJ, Chantalat L, Reynolds CD, Baldwin JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jun 1;61(Pt, 6):541-5. Epub 2005 Jun 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511091 16511091]
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Protein complex]]
[[Category: Protein complex]]
Line 24: Line 33:
[[Category: tetramer-dimer-dimer]]
[[Category: tetramer-dimer-dimer]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:19:11 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:25:29 2008''

Revision as of 12:25, 20 March 2008


PDB ID 1tzy

Drag the structure with the mouse to rotate
, resolution 1.90Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution


Overview

Crystals of native histone octamers (H2A-H2B)-(H4-H3)-(H3'-H4')-(H2B'-H2A') from chick erythrocytes in 2 M KCl, 1.35 M potassium phosphate pH 6.9 diffract X-rays to 1.90 A resolution, yielding a structure with an R(work) value of 18.7% and an Rfree of 22.2%. The crystal space group is P6(5), the asymmetric unit of which contains one complete octamer. This high-resolution model of the histone-core octamer allows further insight into intermolecular interactions, including water molecules, that dock the histone dimers to the tetramer in the nucleosome-core particle and have relevance to nucleosome remodelling. The three key areas analysed are the H2A'-H3-H4 molecular cluster (also H2A-H3'-H4'), the H4-H2B' interaction (also H4'-H2B) and the H2A'-H4 beta-sheet interaction (also H2A-H4'). The latter of these three regions is important to nucleosome remodelling by RNA polymerase II, as it is shown to be a likely core-histone binding site, and its disruption creates an instability in the nucleosome-core particle. A majority of the water molecules in the high-resolution octamer have positions that correlate to similar positions in the high-resolution nucleosome-core particle structure, suggesting that the high-resolution octamer model can be used for comparative studies with the high-resolution nucleosome-core particle.

About this Structure

1TZY is a Protein complex structure of sequences from Gallus gallus. Full crystallographic information is available from OCA.

Reference

High-resolution structure of the native histone octamer., Wood CM, Nicholson JM, Lambert SJ, Chantalat L, Reynolds CD, Baldwin JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jun 1;61(Pt, 6):541-5. Epub 2005 Jun 1. PMID:16511091

Page seeded by OCA on Thu Mar 20 14:25:29 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools