1u1h
From Proteopedia
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| - | [[Image:1u1h.gif|left|200px]] | + | [[Image:1u1h.gif|left|200px]] |
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| - | '''A. thaliana cobalamine independent methionine synthase''' | + | {{Structure |
| + | |PDB= 1u1h |SIZE=350|CAPTION= <scene name='initialview01'>1u1h</scene>, resolution 2.55Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=MET:METHIONINE'>MET</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/5-methyltetrahydropteroyltriglutamate--homocysteine_S-methyltransferase 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.14 2.1.1.14] | ||
| + | |GENE= CIMS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]) | ||
| + | }} | ||
| + | |||
| + | '''A. thaliana cobalamine independent methionine synthase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1U1H is a [ | + | 1U1H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U1H OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of cobalamin-independent methionine synthase complexed with zinc, homocysteine, and methyltetrahydrofolate., Ferrer JL, Ravanel S, Robert M, Dumas R, J Biol Chem. 2004 Oct 22;279(43):44235-8. Epub 2004 Aug 23. PMID:[http:// | + | Crystal structures of cobalamin-independent methionine synthase complexed with zinc, homocysteine, and methyltetrahydrofolate., Ferrer JL, Ravanel S, Robert M, Dumas R, J Biol Chem. 2004 Oct 22;279(43):44235-8. Epub 2004 Aug 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15326182 15326182] |
[[Category: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase]] | [[Category: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase]] | ||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
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[[Category: synthase]] | [[Category: synthase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:26:01 2008'' |
Revision as of 12:26, 20 March 2008
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| , resolution 2.55Å | |||||||
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| Ligands: | , and | ||||||
| Gene: | CIMS (Arabidopsis thaliana) | ||||||
| Activity: | 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase, with EC number 2.1.1.14 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
A. thaliana cobalamine independent methionine synthase
Overview
Cobalamin-independent methionine synthase (MetE) catalyzes the synthesis of methionine by a direct transfer of the methyl group of N5-methyltetrahydrofolate (CH3-H2PteGlun) to the sulfur atom of homocysteine (Hcy). We report here the first crystal structure of this metalloenzyme under different forms, free or complexed with the Hcy and folate substrates. The Arabidopsis thaliana MetE (AtMetE) crystals reveal a monomeric structure built by two (betaalpha)8 barrels making a deep groove at their interface. The active site is located at the surface of the C-terminal domain, facing the large interdomain cleft. Inside the active site, His647, Cys649, and Cys733 are involved in zinc coordination, whereas Asp605, Ile437, and Ser439 interact with Hcy. Opposite the zinc/Hcy binding site, a cationic loop (residues 507-529) belonging to the C-terminal domain anchors the first glutamyl residue of CH3-H4PteGlu5. The pterin moiety of CH3-H4PteGlu5 is stacked with Trp567, enabling the N5-methyl group to protrude in the direction of the zinc atom. These data suggest a structural role of the N-terminal domain of AtMetE in the stabilization of loop 507-529 and in the interaction with the poly-glutamate chain of CH3-H4PteGlun. Comparison of AtMetE structures reveals that the addition of Hcy does not lead to a direct coordination of the sulfur atom with zinc but to a reorganization of the zinc binding site with a stronger coordination to Cys649, Cys733, and a water molecule.
About this Structure
1U1H is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
Crystal structures of cobalamin-independent methionine synthase complexed with zinc, homocysteine, and methyltetrahydrofolate., Ferrer JL, Ravanel S, Robert M, Dumas R, J Biol Chem. 2004 Oct 22;279(43):44235-8. Epub 2004 Aug 23. PMID:15326182
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