1u25

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[[Image:1u25.gif|left|200px]]<br /><applet load="1u25" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1u25.gif|left|200px]]
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caption="1u25, resolution 2.5&Aring;" />
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'''Crystal structure of Selenomonas ruminantium phytase complexed with persulfated phytate in the C2221 crystal form'''<br />
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{{Structure
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|PDB= 1u25 |SIZE=350|CAPTION= <scene name='initialview01'>1u25</scene>, resolution 2.5&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/5-phytase 5-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.72 3.1.3.72]
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|GENE=
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}}
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'''Crystal structure of Selenomonas ruminantium phytase complexed with persulfated phytate in the C2221 crystal form'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1U25 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Selenomonas_ruminantium Selenomonas ruminantium] with <scene name='pdbligand=IHS:'>IHS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/5-phytase 5-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.72 3.1.3.72] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U25 OCA].
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1U25 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Selenomonas_ruminantium Selenomonas ruminantium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U25 OCA].
==Reference==
==Reference==
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Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis., Chu HM, Guo RT, Lin TW, Chou CC, Shr HL, Lai HL, Tang TY, Cheng KJ, Selinger BL, Wang AH, Structure. 2004 Nov;12(11):2015-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15530366 15530366]
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Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis., Chu HM, Guo RT, Lin TW, Chou CC, Shr HL, Lai HL, Tang TY, Cheng KJ, Selinger BL, Wang AH, Structure. 2004 Nov;12(11):2015-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15530366 15530366]
[[Category: 5-phytase]]
[[Category: 5-phytase]]
[[Category: Selenomonas ruminantium]]
[[Category: Selenomonas ruminantium]]
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[[Category: ptp]]
[[Category: ptp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:19:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:26:25 2008''

Revision as of 12:26, 20 March 2008

Image:1u25.gif


PDB ID 1u25

Drag the structure with the mouse to rotate
, resolution 2.5Å
Ligands:
Activity: 5-phytase, with EC number 3.1.3.72
Coordinates: save as pdb, mmCIF, xml



Crystal structure of Selenomonas ruminantium phytase complexed with persulfated phytate in the C2221 crystal form


Overview

Various inositide phosphatases participate in the regulation of inositol polyphosphate signaling molecules. Plant phytases are phosphatases that hydrolyze phytate to less-phosphorylated myo-inositol derivatives and phosphate. The phytase from Selenomonas ruminantium shares no sequence homology with other microbial phytases. Its crystal structure revealed a phytase fold of the dual-specificity phosphatase type. The active site is located near a conserved cysteine-containing (Cys241) P loop. We also solved two other crystal forms in which an inhibitor, myo-inositol hexasulfate, is cocrystallized with the enzyme. In the "standby" and the "inhibited" crystal forms, the inhibitor is bound, respectively, in a pocket slightly away from Cys241 and at the substrate binding site where the phosphate group to be hydrolyzed is held close to the -SH group of Cys241. Our structural and mutagenesis studies allow us to visualize the way in which the P loop-containing phytase attracts and hydrolyzes the substrate (phytate) sequentially.

About this Structure

1U25 is a Single protein structure of sequence from Selenomonas ruminantium. Full crystallographic information is available from OCA.

Reference

Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis., Chu HM, Guo RT, Lin TW, Chou CC, Shr HL, Lai HL, Tang TY, Cheng KJ, Selinger BL, Wang AH, Structure. 2004 Nov;12(11):2015-24. PMID:15530366

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