1u2f
From Proteopedia
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| - | [[Image:1u2f.gif|left|200px]] | + | [[Image:1u2f.gif|left|200px]] |
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| - | '''SOLUTION STRUCTURE OF THE FIRST RNA-BINDING DOMAIN OF HU2AF65''' | + | {{Structure |
| + | |PDB= 1u2f |SIZE=350|CAPTION= <scene name='initialview01'>1u2f</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''SOLUTION STRUCTURE OF THE FIRST RNA-BINDING DOMAIN OF HU2AF65''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1U2F is a [ | + | 1U2F is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U2F OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structures of the first and second RNA-binding domains of human U2 small nuclear ribonucleoprotein particle auxiliary factor (U2AF(65))., Ito T, Muto Y, Green MR, Yokoyama S, EMBO J. 1999 Aug 16;18(16):4523-34. PMID:[http:// | + | Solution structures of the first and second RNA-binding domains of human U2 small nuclear ribonucleoprotein particle auxiliary factor (U2AF(65))., Ito T, Muto Y, Green MR, Yokoyama S, EMBO J. 1999 Aug 16;18(16):4523-34. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10449418 10449418] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: rsgi]] | [[Category: rsgi]] | ||
[[Category: splicing]] | [[Category: splicing]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
[[Category: u2 snrnp]] | [[Category: u2 snrnp]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:26:30 2008'' |
Revision as of 12:26, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF THE FIRST RNA-BINDING DOMAIN OF HU2AF65
Overview
The large subunit of the human U2 small nuclear ribonucleoprotein particle auxiliary factor (hU2AF(65)) is an essential RNA-splicing factor required for the recognition of the polypyrimidine tract immediately upstream of the 3' splice site. In the present study, we determined the solution structures of two hU2AF(65) fragments, corresponding to the first and second RNA-binding domains (RBD1 and RBD2, respectively), by nuclear magnetic resonance spectroscopy. The tertiary structure of RBD2 is similar to that of typical RNA-binding domains with the beta1-alpha1-beta2-beta3-alpha2-beta4 topology. In contrast, the hU2AF(65) RBD1 structure has unique features: (i) the alpha1 helix is elongated by one turn toward the C-terminus; (ii) the loop between alpha1 and beta2 (the alpha1/beta2 loop) is much longer and has a defined conformation; (iii) the beta2 strand is (188)AVQIN(192), which was not predicted by sequence alignments; and (iv) the beta2/beta3 loop is much shorter. Chemical shift perturbation experiments showed that the U2AF-binding RNA fragments interact with the four beta-strands of RBD2 whereas, in contrast, they interact with beta1, beta3 and beta4, but not with beta2 or the alpha1/beta2 loop, of RBD1. The characteristic alpha1-beta2 structure of the hU2AF(65) RBD1 may interact with other proteins, such as UAP56.
About this Structure
1U2F is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structures of the first and second RNA-binding domains of human U2 small nuclear ribonucleoprotein particle auxiliary factor (U2AF(65))., Ito T, Muto Y, Green MR, Yokoyama S, EMBO J. 1999 Aug 16;18(16):4523-34. PMID:10449418
Page seeded by OCA on Thu Mar 20 14:26:30 2008
