1u6j
From Proteopedia
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| - | [[Image:1u6j.gif|left|200px]] | + | [[Image:1u6j.gif|left|200px]] |
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| - | '''The Structure of native coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase at 2.4A resolution''' | + | {{Structure |
| + | |PDB= 1u6j |SIZE=350|CAPTION= <scene name='initialview01'>1u6j</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Methylenetetrahydromethanopterin_dehydrogenase Methylenetetrahydromethanopterin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.9 1.5.99.9] | ||
| + | |GENE= mtd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2320 Methanopyrus kandleri]) | ||
| + | }} | ||
| + | |||
| + | '''The Structure of native coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase at 2.4A resolution''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1U6J is a [ | + | 1U6J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanopyrus_kandleri Methanopyrus kandleri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6J OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of F420-dependent methylenetetrahydromethanopterin dehydrogenase: a crystallographic 'superstructure' of the selenomethionine-labelled protein crystal structure., Warkentin E, Hagemeier CH, Shima S, Thauer RK, Ermler U, Acta Crystallogr D Biol Crystallogr. 2005 Feb;61(Pt 2):198-202. Epub 2005, Jan 19. PMID:[http:// | + | The structure of F420-dependent methylenetetrahydromethanopterin dehydrogenase: a crystallographic 'superstructure' of the selenomethionine-labelled protein crystal structure., Warkentin E, Hagemeier CH, Shima S, Thauer RK, Ermler U, Acta Crystallogr D Biol Crystallogr. 2005 Feb;61(Pt 2):198-202. Epub 2005, Jan 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15681872 15681872] |
[[Category: Methanopyrus kandleri]] | [[Category: Methanopyrus kandleri]] | ||
[[Category: Methylenetetrahydromethanopterin dehydrogenase]] | [[Category: Methylenetetrahydromethanopterin dehydrogenase]] | ||
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[[Category: Warkentin, E.]] | [[Category: Warkentin, E.]] | ||
[[Category: MG]] | [[Category: MG]] | ||
| - | [[Category: helix bundle; trimer of | + | [[Category: helix bundle; trimer of dimer]] |
[[Category: monomer: alpha/beta domain]] | [[Category: monomer: alpha/beta domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:27:54 2008'' |
Revision as of 12:27, 20 March 2008
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| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | mtd (Methanopyrus kandleri) | ||||||
| Activity: | Methylenetetrahydromethanopterin dehydrogenase, with EC number 1.5.99.9 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The Structure of native coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase at 2.4A resolution
Overview
The diffraction pattern of native protein crystals of F(420)-dependent methylenetetrahydromethanopterin dehydrogenase from Methanopyrus kandleri shows weak additional reflections compared with the selenomethionine-labelled protein crystals, indicating a doubled c unit-cell parameter. These reflections indicate small reorientations of the hexameric structural units, breaking the translational symmetry. TLS refinement of the selenomethionine-labelled protein structure at 1.55 A resolution revealed an anisotropic rigid-body libration of the hexameric units. The anisotropy is consistent with the static reorientation in the native protein crystals. These results are discussed as related to the crystal packing. The relation between the two structures suggests an analogy to structural changes during certain kinds of phase transitions that have been well studied in inorganic structural chemistry.
About this Structure
1U6J is a Single protein structure of sequence from Methanopyrus kandleri. Full crystallographic information is available from OCA.
Reference
The structure of F420-dependent methylenetetrahydromethanopterin dehydrogenase: a crystallographic 'superstructure' of the selenomethionine-labelled protein crystal structure., Warkentin E, Hagemeier CH, Shima S, Thauer RK, Ermler U, Acta Crystallogr D Biol Crystallogr. 2005 Feb;61(Pt 2):198-202. Epub 2005, Jan 19. PMID:15681872
Page seeded by OCA on Thu Mar 20 14:27:54 2008
