1u73
From Proteopedia
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| - | [[Image:1u73.gif|left|200px]] | + | [[Image:1u73.gif|left|200px]] |
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| - | '''Crystal structure of a Dimeric Acidic Platelet Aggregation Inhibitor and Hypotensive Phospholipase A2 from Bothrops jararacussu''' | + | {{Structure |
| + | |PDB= 1u73 |SIZE=350|CAPTION= <scene name='initialview01'>1u73</scene>, resolution 1.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of a Dimeric Acidic Platelet Aggregation Inhibitor and Hypotensive Phospholipase A2 from Bothrops jararacussu''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1U73 is a [ | + | 1U73 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U73 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of an acidic platelet aggregation inhibitor and hypotensive phospholipase A2 in the monomeric and dimeric states: insights into its oligomeric state., Magro AJ, Murakami MT, Marcussi S, Soares AM, Arni RK, Fontes MR, Biochem Biophys Res Commun. 2004 Oct 8;323(1):24-31. PMID:[http:// | + | Crystal structure of an acidic platelet aggregation inhibitor and hypotensive phospholipase A2 in the monomeric and dimeric states: insights into its oligomeric state., Magro AJ, Murakami MT, Marcussi S, Soares AM, Arni RK, Fontes MR, Biochem Biophys Res Commun. 2004 Oct 8;323(1):24-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15351695 15351695] |
[[Category: Bothrops jararacussu]] | [[Category: Bothrops jararacussu]] | ||
[[Category: Phospholipase A(2)]] | [[Category: Phospholipase A(2)]] | ||
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[[Category: dimeric]] | [[Category: dimeric]] | ||
[[Category: oligomeric state]] | [[Category: oligomeric state]] | ||
| - | [[Category: platelet aggregation and hypotensive | + | [[Category: platelet aggregation and hypotensive effect]] |
[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:28:05 2008'' |
Revision as of 12:28, 20 March 2008
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| , resolution 1.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Phospholipase A(2), with EC number 3.1.1.4 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a Dimeric Acidic Platelet Aggregation Inhibitor and Hypotensive Phospholipase A2 from Bothrops jararacussu
Overview
Phospholipases A2 belong to the superfamily of proteins which hydrolyzes the sn-2 acyl groups of membrane phospholipids to release arachidonic acid and lysophospholipids. An acidic phospholipase A2 isolated from Bothrops jararacussu snake venom presents a high catalytic, platelet aggregation inhibition and hypotensive activities. This protein was crystallized in two oligomeric states: monomeric and dimeric. The crystal structures were solved at 1.79 and 1.90 angstroms resolution, respectively, for the two states. It was identified a Na+ ion at the center of Ca2+-binding site of the monomeric form. A novel dimeric conformation with the active sites exposed to the solvent was observed. Conformational states of the molecule may be due to the physicochemical conditions used in the crystallization experiments. We suggest dimeric state is one found in vivo.
About this Structure
1U73 is a Single protein structure of sequence from Bothrops jararacussu. Full crystallographic information is available from OCA.
Reference
Crystal structure of an acidic platelet aggregation inhibitor and hypotensive phospholipase A2 in the monomeric and dimeric states: insights into its oligomeric state., Magro AJ, Murakami MT, Marcussi S, Soares AM, Arni RK, Fontes MR, Biochem Biophys Res Commun. 2004 Oct 8;323(1):24-31. PMID:15351695
Page seeded by OCA on Thu Mar 20 14:28:05 2008
Categories: Bothrops jararacussu | Phospholipase A(2) | Single protein | Arni, R K. | Fontes, M R. | Magro, A J. | Murakami, M T. | Soares, A M. | Acidic phospholipase a2 | Bothrops jararacussu venom | Crystal structure | Dimeric | Oligomeric state | Platelet aggregation and hypotensive effect | X-ray crystallography
