1u7m
From Proteopedia
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| - | [[Image:1u7m.gif|left|200px]] | + | [[Image:1u7m.gif|left|200px]] |
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| - | '''Solution structure of a diiron protein model: Due Ferri(II) turn mutant''' | + | {{Structure |
| + | |PDB= 1u7m |SIZE=350|CAPTION= <scene name='initialview01'>1u7m</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of a diiron protein model: Due Ferri(II) turn mutant''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1U7M is a [ | + | 1U7M is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7M OCA]. |
==Reference== | ==Reference== | ||
| - | Analysis and design of turns in alpha-helical hairpins., Lahr SJ, Engel DE, Stayrook SE, Maglio O, North B, Geremia S, Lombardi A, DeGrado WF, J Mol Biol. 2005 Mar 11;346(5):1441-54. Epub 2005 Jan 13. PMID:[http:// | + | Analysis and design of turns in alpha-helical hairpins., Lahr SJ, Engel DE, Stayrook SE, Maglio O, North B, Geremia S, Lombardi A, DeGrado WF, J Mol Biol. 2005 Mar 11;346(5):1441-54. Epub 2005 Jan 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713492 15713492] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: Pavone, V.]] | [[Category: Pavone, V.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| - | [[Category: diiron | + | [[Category: diiron protein]] |
[[Category: four-helix bundle]] | [[Category: four-helix bundle]] | ||
| - | [[Category: inter-helical | + | [[Category: inter-helical loop]] |
[[Category: protein design]] | [[Category: protein design]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:28:22 2008'' |
Revision as of 12:28, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of a diiron protein model: Due Ferri(II) turn mutant
Overview
Although the analysis and design of turns that connect the strands in antiparallel beta-hairpins has reached an advanced state, much less is known concerning turns between antiparallel helices in helical hairpins. We have conducted an analysis of the structures and sequence preferences of two types of interhelical turns, each of which connects the two helices by a two-residue linker in an alphaL-beta conformation. Based on this analysis, it became apparent that the turn introduced into a designed four-helix bundle protein, DF1, did not occur within an optimal structural context. DF1 is a dimeric model for the diiron class of proteins. A longer loop with a beta-alphaR-beta conformation was inserted between two helices in the protein, and a sequence was chosen to stabilize its conformation. X-ray crystallography and NMR analysis of the protein showed the structure to be in excellent agreement with design.
About this Structure
1U7M is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Analysis and design of turns in alpha-helical hairpins., Lahr SJ, Engel DE, Stayrook SE, Maglio O, North B, Geremia S, Lombardi A, DeGrado WF, J Mol Biol. 2005 Mar 11;346(5):1441-54. Epub 2005 Jan 13. PMID:15713492
Page seeded by OCA on Thu Mar 20 14:28:22 2008
