1u90
From Proteopedia
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| - | [[Image:1u90.jpg|left|200px]] | + | [[Image:1u90.jpg|left|200px]] |
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| - | '''Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap''' | + | {{Structure |
| + | |PDB= 1u90 |SIZE=350|CAPTION= <scene name='initialview01'>1u90</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= Rala, Ral-a, Ral ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9490 Saguinus oedipus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1U90 is a [ | + | 1U90 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saguinus_oedipus Saguinus oedipus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U90 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of Ral-GppNHp and Ral-GDP reveal two binding sites that are also present in Ras and Rap., Nicely NI, Kosak J, de Serrano V, Mattos C, Structure. 2004 Nov;12(11):2025-36. PMID:[http:// | + | Crystal structures of Ral-GppNHp and Ral-GDP reveal two binding sites that are also present in Ras and Rap., Nicely NI, Kosak J, de Serrano V, Mattos C, Structure. 2004 Nov;12(11):2025-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15530367 15530367] |
[[Category: Saguinus oedipus]] | [[Category: Saguinus oedipus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: gtpase]] | [[Category: gtpase]] | ||
[[Category: ral]] | [[Category: ral]] | ||
| - | [[Category: | + | [[Category: ra]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:28:53 2008'' |
Revision as of 12:28, 20 March 2008
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| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | Rala, Ral-a, Ral (Saguinus oedipus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap
Overview
RalA is a GTPase with effectors such as Sec5 and Exo84 in the exocyst complex and RalBP1, a GAP for Rho proteins. We report the crystal structures of Ral-GppNHp and Ral-GDP. Disordered switch I and switch II, located away from crystal contacts, are observed in one of the molecules in the asymmetric unit of the Ral-GppNHp structure. In the other molecule in the asymmetric unit, a second Mg(2+) ion is bound to the GppNHp gamma-phosphate in an environment in which switch I is pulled away from the nucleotide and switch II is found in a tight beta turn. Clustering of conserved residues on the surface of Ral-GppNHp identifies two putative sites for protein-protein interaction. One site is adjacent to switch I. The other is modulated by switch II and is obstructed in Ral-GDP. The Ral structures are discussed in the context of the published structures of the Ral/Sec5 complex, Ras, and Rap.
About this Structure
1U90 is a Single protein structure of sequence from Saguinus oedipus. Full crystallographic information is available from OCA.
Reference
Crystal structures of Ral-GppNHp and Ral-GDP reveal two binding sites that are also present in Ras and Rap., Nicely NI, Kosak J, de Serrano V, Mattos C, Structure. 2004 Nov;12(11):2025-36. PMID:15530367
Page seeded by OCA on Thu Mar 20 14:28:53 2008
Categories: Saguinus oedipus | Single protein | Kosak, J. | Mattos, C. | Nicely, N I. | Serrano, V de. | GDP | MG | Gdp | Gmppnp | Gnp | Gppnhp | Gtp | Gtpase | Ral | Ra
