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1uas
From Proteopedia
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| - | [[Image:1uas.jpg|left|200px]] | + | [[Image:1uas.jpg|left|200px]] |
| - | + | ||
| - | '''Crystal structure of rice alpha-galactosidase''' | + | {{Structure |
| + | |PDB= 1uas |SIZE=350|CAPTION= <scene name='initialview01'>1uas</scene>, resolution 1.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of rice alpha-galactosidase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UAS is a [ | + | 1UAS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UAS OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of rice alpha-galactosidase complexed with D-galactose., Fujimoto Z, Kaneko S, Momma M, Kobayashi H, Mizuno H, J Biol Chem. 2003 May 30;278(22):20313-8. Epub 2003 Mar 25. PMID:[http:// | + | Crystal structure of rice alpha-galactosidase complexed with D-galactose., Fujimoto Z, Kaneko S, Momma M, Kobayashi H, Mizuno H, J Biol Chem. 2003 May 30;278(22):20313-8. Epub 2003 Mar 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12657636 12657636] |
[[Category: Alpha-galactosidase]] | [[Category: Alpha-galactosidase]] | ||
[[Category: Oryza sativa]] | [[Category: Oryza sativa]] | ||
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[[Category: tim-barrel]] | [[Category: tim-barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:29:31 2008'' |
Revision as of 12:29, 20 March 2008
| |||||||
| , resolution 1.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Activity: | Alpha-galactosidase, with EC number 3.2.1.22 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of rice alpha-galactosidase
Overview
alpha-Galactosidases catalyze the hydrolysis of alpha-1,6-linked galactosyl residues from galacto-oligosaccharides and polymeric galacto-(gluco)mannans. The crystal structure of rice alpha-galactosidase has been determined at 1.5A resolution using the multiple isomorphous replacement method. The structure consisted of a catalytic domain and a C-terminal domain and was essentially the same as that of alpha-N-acetylgalactosaminidase, which is the same member of glycosyl hydrolase family 27. The catalytic domain had a (beta/alpha)8-barrel structure, and the C-terminal domain was made up of eight beta-strands containing a Greek key motif. The structure was solved as a complex with d-galactose, providing a mode of substrate binding in detail. The d-galactose molecule was found bound in the active site pocket on the C-terminal side of the central beta-barrel of the catalytic domain. The d-galactose molecule consisted of a mixture of two anomers present in a ratio equal to their natural abundance. Structural comparisons of rice alpha-galactosidase with chicken alpha-N-acetylgalactosaminidase provided further understanding of the substrate recognition mechanism in these enzymes.
About this Structure
1UAS is a Single protein structure of sequence from Oryza sativa. Full crystallographic information is available from OCA.
Reference
Crystal structure of rice alpha-galactosidase complexed with D-galactose., Fujimoto Z, Kaneko S, Momma M, Kobayashi H, Mizuno H, J Biol Chem. 2003 May 30;278(22):20313-8. Epub 2003 Mar 25. PMID:12657636
Page seeded by OCA on Thu Mar 20 14:29:31 2008
Categories: Alpha-galactosidase | Oryza sativa | Single protein | Fujimoto, Z. | Kaneko, S. | Kobayashi, H. | Mizuno, H. | Momma, M. | GLA | GOL | PT | SO4 | Beta-alpha-barrel | Greek key motif | Tim-barrel
