1ue3

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[[Image:1ue3.gif|left|200px]]<br /><applet load="1ue3" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ue3.gif|left|200px]]
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caption="1ue3, resolution 2.15&Aring;" />
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'''Crystal structure of d(GCGAAAGC) containing hexaamminecobalt'''<br />
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{{Structure
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|PDB= 1ue3 |SIZE=350|CAPTION= <scene name='initialview01'>1ue3</scene>, resolution 2.15&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=NCO:COBALT HEXAMMINE ION'>NCO</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal structure of d(GCGAAAGC) containing hexaamminecobalt'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1UE3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=NCO:'>NCO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UE3 OCA].
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1UE3 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UE3 OCA].
==Reference==
==Reference==
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Structure of d(GCGAAAGC) (hexagonal form): a base-intercalated duplex as a stable structure., Sunami T, Kondo J, Hirao I, Watanabe K, Miura KI, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):90-6. Epub 2003 Dec, 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14684897 14684897]
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Structure of d(GCGAAAGC) (hexagonal form): a base-intercalated duplex as a stable structure., Sunami T, Kondo J, Hirao I, Watanabe K, Miura KI, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):90-6. Epub 2003 Dec, 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14684897 14684897]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Hirao, I.]]
[[Category: Hirao, I.]]
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[[Category: zipper-like duplex]]
[[Category: zipper-like duplex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:23:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:30:47 2008''

Revision as of 12:30, 20 March 2008

Image:1ue3.gif


PDB ID 1ue3

Drag the structure with the mouse to rotate
, resolution 2.15Å
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



Crystal structure of d(GCGAAAGC) containing hexaamminecobalt


Overview

A DNA fragment d(GCGAAAGC), postulated to adopt a stable mini-hairpin structure on the basis of its extraordinary properties, has been X-ray analyzed. Two octamers related by a crystallographic twofold symmetry are aligned in an antiparallel fashion and associate to form a duplex, which is maintained by two Watson-Crick G.C base pairs and a subsequent sheared G.A pair at both ends. The central two A residues are free from base-pair formation. The corresponding base moieties of the two strands are intercalated and stacked on each other, forming a long column of G(1)-C(2)-G(3)-A(4)-A(5)(*)-A(5)-A(4)(*)-G(3)(*)-C(2)(*)-G(1)(*) (asterisks indicate the counter-strand). The Watson-Crick and major-groove sites of the four stacked adenine bases are exposed to the solvent region, suggesting a functional role. Since this structural motif is similar to those found in the nonamers d(G(Br)CGAAAGCT) and d(G(I)CGAAAGCT), the base-intercalated duplex may be a stable form of the specific sequence. Electrophoresis results suggest that the octamer has two states, monomeric and dimeric, in solution depending on the Mg(2+) concentration. The present duplex is preferred under the crystallization conditions, which correspond to physiologically allowed conditions.

About this Structure

1UE3 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Structure of d(GCGAAAGC) (hexagonal form): a base-intercalated duplex as a stable structure., Sunami T, Kondo J, Hirao I, Watanabe K, Miura KI, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):90-6. Epub 2003 Dec, 18. PMID:14684897

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