1f61

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[[Image:1f61.png|left|200px]]
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==CRYSTAL STRUCTURE OF ISOCITRATE LYASE FROM MYCOBACTERIUM TUBERCULOSIS==
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<StructureSection load='1f61' size='340' side='right' caption='[[1f61]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1f61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F61 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isocitrate_lyase Isocitrate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.1 4.1.3.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f61 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1f61 RCSB], [http://www.ebi.ac.uk/pdbsum/1f61 PDBsum], [http://www.topsan.org/Proteins/TBSGC/1f61 TOPSAN]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f6/1f61_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Isocitrate lyase (ICL) plays a pivotal role in the persistence of Mycobacterium tuberculosis in mice by sustaining intracellular infection in inflammatory macrophages. The enzyme allows net carbon gain by diverting acetyl-CoA from beta-oxidation of fatty acids into the glyoxylate shunt pathway. Given its potential as a drug target against persistent infections, we solved its structure without ligand and in complex with two inhibitors. Covalent modification of an active site residue, Cys 191, by the inhibitor 3-bromopyruvate traps the enzyme in a catalytic conformation with the active site completely inaccessible to solvent. The structure of a C191S mutant of the enzyme with the inhibitor 3-nitropropionate provides further insight into the reaction mechanism.
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{{STRUCTURE_1f61| PDB=1f61 | SCENE= }}
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Structure of isocitrate lyase, a persistence factor of Mycobacterium tuberculosis.,Sharma V, Sharma S, Hoener zu Bentrup K, McKinney JD, Russell DG, Jacobs WR Jr, Sacchettini JC Nat Struct Biol. 2000 Aug;7(8):663-8. PMID:10932251<ref>PMID:10932251</ref>
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===CRYSTAL STRUCTURE OF ISOCITRATE LYASE FROM MYCOBACTERIUM TUBERCULOSIS===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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==About this Structure==
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<references/>
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[[1f61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F61 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:010932251</ref><ref group="xtra">PMID:010966645</ref><references group="xtra"/>
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[[Category: Isocitrate lyase]]
[[Category: Isocitrate lyase]]
[[Category: Mycobacterium tuberculosis h37rv]]
[[Category: Mycobacterium tuberculosis h37rv]]
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[[Category: Bentrup, K H.Hoener zu.]]
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[[Category: Bentrup, K H.Hoener zu]]
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[[Category: Jacobs, W R.]]
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[[Category: Jacobs, W R]]
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[[Category: McKinney, J D.]]
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[[Category: McKinney, J D]]
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[[Category: Russell, D G.]]
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[[Category: Russell, D G]]
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[[Category: Sacchettini, J C.]]
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[[Category: Sacchettini, J C]]
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[[Category: Sharma, S.]]
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[[Category: Sharma, S]]
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[[Category: Sharma, V.]]
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[[Category: Sharma, V]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: Structural genomic]]
[[Category: Alpha-beta barrel]]
[[Category: Alpha-beta barrel]]
[[Category: Apo-enzyme]]
[[Category: Apo-enzyme]]
[[Category: Lyase]]
[[Category: Lyase]]
[[Category: Open conformation]]
[[Category: Open conformation]]
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[[Category: Protein structure initiative]]
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[[Category: PSI, Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Structural genomic]]
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[[Category: Swapped helice]]
[[Category: Swapped helice]]
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[[Category: Tb structural genomics consortium]]
 
[[Category: Tbsgc]]
[[Category: Tbsgc]]

Revision as of 18:51, 22 December 2014

CRYSTAL STRUCTURE OF ISOCITRATE LYASE FROM MYCOBACTERIUM TUBERCULOSIS

1f61, resolution 2.00Å

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