1ujw

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[[Image:1ujw.gif|left|200px]]<br /><applet load="1ujw" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ujw.gif|left|200px]]
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caption="1ujw, resolution 2.75&Aring;" />
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'''Structure of the complex between BtuB and Colicin E3 Receptor binding domain'''<br />
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{{Structure
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|PDB= 1ujw |SIZE=350|CAPTION= <scene name='initialview01'>1ujw</scene>, resolution 2.75&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=GP1:GLUCOSAMINE+1-PHOSPHATE'>GP1</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=AAE:ACETOACETIC+ACID'>AAE</scene>, <scene name='pdbligand=LIM:3-OXO-PENTADECANOIC+ACID'>LIM</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Structure of the complex between BtuB and Colicin E3 Receptor binding domain'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1UJW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=GP1:'>GP1</scene>, <scene name='pdbligand=LDA:'>LDA</scene>, <scene name='pdbligand=AAE:'>AAE</scene>, <scene name='pdbligand=LIM:'>LIM</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UJW OCA].
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1UJW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UJW OCA].
==Reference==
==Reference==
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The structure of BtuB with bound colicin E3 R-domain implies a translocon., Kurisu G, Zakharov SD, Zhalnina MV, Bano S, Eroukova VY, Rokitskaya TI, Antonenko YN, Wiener MC, Cramer WA, Nat Struct Biol. 2003 Nov;10(11):948-54. Epub 2003 Oct 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14528295 14528295]
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The structure of BtuB with bound colicin E3 R-domain implies a translocon., Kurisu G, Zakharov SD, Zhalnina MV, Bano S, Eroukova VY, Rokitskaya TI, Antonenko YN, Wiener MC, Cramer WA, Nat Struct Biol. 2003 Nov;10(11):948-54. Epub 2003 Oct 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14528295 14528295]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: coiled-coil]]
[[Category: coiled-coil]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:25:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:32:54 2008''

Revision as of 12:33, 20 March 2008

Image:1ujw.gif


PDB ID 1ujw

Drag the structure with the mouse to rotate
, resolution 2.75Å
Ligands: , , , and
Coordinates: save as pdb, mmCIF, xml



Structure of the complex between BtuB and Colicin E3 Receptor binding domain


Overview

Cellular import of colicin E3 is initiated by the Escherichia coli outer membrane cobalamin transporter, BtuB. The 135-residue 100-A coiled-coil receptor-binding domain (R135) of colicin E3 forms a 1:1 complex with BtuB whose structure at a resolution of 2.75 A is reported. Binding of R135 to the BtuB extracellular surface (DeltaG(o) = -12 kcal mol(-1)) is mediated by 27 residues of R135 near the coiled-coil apex. Formation of the R135-BtuB complex results in unfolding of R135 N- and C-terminal ends, inferred to be important for unfolding of the colicin T-domain. Small conformational changes occur in the BtuB cork and barrel domains but are insufficient to form a translocation channel. The absence of a channel and the peripheral binding of R135 imply that BtuB serves to bind the colicin, and that the coiled-coil delivers the colicin to a neighboring outer membrane protein for translocation, thus forming a colicin translocon. The translocator was concluded to be OmpF from the occlusion of OmpF channels by colicin E3.

About this Structure

1UJW is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The structure of BtuB with bound colicin E3 R-domain implies a translocon., Kurisu G, Zakharov SD, Zhalnina MV, Bano S, Eroukova VY, Rokitskaya TI, Antonenko YN, Wiener MC, Cramer WA, Nat Struct Biol. 2003 Nov;10(11):948-54. Epub 2003 Oct 5. PMID:14528295

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