1uoc
From Proteopedia
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| - | [[Image:1uoc.gif|left|200px]] | + | [[Image:1uoc.gif|left|200px]] |
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| - | '''X-RAY STRUCTURE OF THE RNASE DOMAIN OF THE YEAST POP2 PROTEIN''' | + | {{Structure |
| + | |PDB= 1uoc |SIZE=350|CAPTION= <scene name='initialview01'>1uoc</scene>, resolution 2.30Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Environment+For+Xe+In+Chain+B'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=XE:XENON'>XE</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''X-RAY STRUCTURE OF THE RNASE DOMAIN OF THE YEAST POP2 PROTEIN''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UOC is a [ | + | 1UOC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UOC OCA]. |
==Reference== | ==Reference== | ||
| - | X-ray structure and activity of the yeast Pop2 protein: a nuclease subunit of the mRNA deadenylase complex., Thore S, Mauxion F, Seraphin B, Suck D, EMBO Rep. 2003 Dec;4(12):1150-5. Epub 2003 Nov 14. PMID:[http:// | + | X-ray structure and activity of the yeast Pop2 protein: a nuclease subunit of the mRNA deadenylase complex., Thore S, Mauxion F, Seraphin B, Suck D, EMBO Rep. 2003 Dec;4(12):1150-5. Epub 2003 Nov 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14618157 14618157] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:34:38 2008'' |
Revision as of 12:34, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-RAY STRUCTURE OF THE RNASE DOMAIN OF THE YEAST POP2 PROTEIN
Overview
In Saccharomyces cerevisiae, a large complex, known as the Ccr4-Not complex, containing two nucleases, is responsible for mRNA deadenylation. One of these nucleases is called Pop2 and has been identified by similarity with PARN, a human poly(A) nuclease. Here, we present the crystal structure of the nuclease domain of Pop2 at 2.3 A resolution. The domain has the fold of the DnaQ family and represents the first structure of an RNase from the DEDD superfamily. Despite the presence of two non-canonical residues in the active site, the domain displays RNase activity on a broad range of RNA substrates. Site-directed mutagenesis of active-site residues demonstrates the intrinsic ability of the Pop2 RNase D domain to digest RNA. This first structure of a nuclease involved in the 3'-5' deadenylation of mRNA in yeast provides information for the understanding of the mechanism by which the Ccr4-Not complex achieves its functions.
About this Structure
1UOC is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
X-ray structure and activity of the yeast Pop2 protein: a nuclease subunit of the mRNA deadenylase complex., Thore S, Mauxion F, Seraphin B, Suck D, EMBO Rep. 2003 Dec;4(12):1150-5. Epub 2003 Nov 14. PMID:14618157
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