1uqr

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[[Image:1uqr.gif|left|200px]]<br /><applet load="1uqr" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1uqr.gif|left|200px]]
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caption="1uqr, resolution 1.7&Aring;" />
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'''TYPE II 3-DEHYDROQUINATE DEHYDRATASE (DHQASE) FROM ACTINOBACILLUS PLEUROPNEUMONIAE'''<br />
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{{Structure
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|PDB= 1uqr |SIZE=350|CAPTION= <scene name='initialview01'>1uqr</scene>, resolution 1.7&Aring;
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|SITE= <scene name='pdbsite=AC1:Trs+Binding+Site+For+Chain+K'>AC1</scene>
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/3-dehydroquinate_dehydratase 3-dehydroquinate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.10 4.2.1.10]
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|GENE=
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}}
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'''TYPE II 3-DEHYDROQUINATE DEHYDRATASE (DHQASE) FROM ACTINOBACILLUS PLEUROPNEUMONIAE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1UQR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Actinobacillus_pleuropneumoniae Actinobacillus pleuropneumoniae] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-dehydroquinate_dehydratase 3-dehydroquinate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.10 4.2.1.10] Known structural/functional Site: <scene name='pdbsite=AC1:Trs+Binding+Site+For+Chain+K'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UQR OCA].
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1UQR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Actinobacillus_pleuropneumoniae Actinobacillus pleuropneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UQR OCA].
==Reference==
==Reference==
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Structural study of the type II 3-dehydroquinate dehydratase from Actinobacillus pleuropneumoniae., Maes D, Gonzalez-Ramirez LA, Lopez-Jaramillo J, Yu B, De Bondt H, Zegers I, Afonina E, Garcia-Ruiz JM, Gulnik S, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):463-71. Epub 2004, Feb 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14993670 14993670]
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Structural study of the type II 3-dehydroquinate dehydratase from Actinobacillus pleuropneumoniae., Maes D, Gonzalez-Ramirez LA, Lopez-Jaramillo J, Yu B, De Bondt H, Zegers I, Afonina E, Garcia-Ruiz JM, Gulnik S, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):463-71. Epub 2004, Feb 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14993670 14993670]
[[Category: 3-dehydroquinate dehydratase]]
[[Category: 3-dehydroquinate dehydratase]]
[[Category: Actinobacillus pleuropneumoniae]]
[[Category: Actinobacillus pleuropneumoniae]]
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[[Category: shikimate pathway]]
[[Category: shikimate pathway]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:27:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:35:27 2008''

Revision as of 12:35, 20 March 2008

Image:1uqr.gif


PDB ID 1uqr

Drag the structure with the mouse to rotate
, resolution 1.7Å
Sites:
Ligands: and
Activity: 3-dehydroquinate dehydratase, with EC number 4.2.1.10
Coordinates: save as pdb, mmCIF, xml



TYPE II 3-DEHYDROQUINATE DEHYDRATASE (DHQASE) FROM ACTINOBACILLUS PLEUROPNEUMONIAE


Overview

The structure of the type II dehydroquinate dehydratase (DHQase) from Actinobacillus pleuropneumoniae, the third enzyme of the shikimate pathway, has been determined. Crystals diffracting to 1.7 A were obtained in space and on earth using the counter-diffusion technique. The structure was solved using molecular replacement and refined to high resolution. The overall structure of the dodecameric enzyme is described and compared with structures of DHQases from other bacteria. DHQases contain a flexible loop that presumably closes over the active site upon substrate binding. The enzyme can exist in an open or closed conformation. The present structure displays the open conformation, with a sulfate anion bound in the active site. The availability of this structure opens a route to structure-based antibiotics targetting this pathogenic bacterium.

About this Structure

1UQR is a Single protein structure of sequence from Actinobacillus pleuropneumoniae. Full crystallographic information is available from OCA.

Reference

Structural study of the type II 3-dehydroquinate dehydratase from Actinobacillus pleuropneumoniae., Maes D, Gonzalez-Ramirez LA, Lopez-Jaramillo J, Yu B, De Bondt H, Zegers I, Afonina E, Garcia-Ruiz JM, Gulnik S, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):463-71. Epub 2004, Feb 25. PMID:14993670

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