1ur6
From Proteopedia
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| - | [[Image:1ur6.gif|left|200px]] | + | [[Image:1ur6.gif|left|200px]] |
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| - | '''NMR BASED STRUCTURAL MODEL OF THE UBCH5B-CNOT4 COMPLEX''' | + | {{Structure |
| + | |PDB= 1ur6 |SIZE=350|CAPTION= <scene name='initialview01'>1ur6</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''NMR BASED STRUCTURAL MODEL OF THE UBCH5B-CNOT4 COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1UR6 is a [ | + | 1UR6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UR6 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural model of the UbcH5B/CNOT4 complex revealed by combining NMR, mutagenesis, and docking approaches., Dominguez C, Bonvin AM, Winkler GS, van Schaik FM, Timmers HT, Boelens R, Structure. 2004 Apr;12(4):633-44. PMID:[http:// | + | Structural model of the UbcH5B/CNOT4 complex revealed by combining NMR, mutagenesis, and docking approaches., Dominguez C, Bonvin AM, Winkler GS, van Schaik FM, Timmers HT, Boelens R, Structure. 2004 Apr;12(4):633-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15062086 15062086] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: ubiquitin ligase]] | [[Category: ubiquitin ligase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:35:35 2008'' |
Revision as of 12:35, 20 March 2008
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| Ligands: | |||||||
| Activity: | Ubiquitin--protein ligase, with EC number 6.3.2.19 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR BASED STRUCTURAL MODEL OF THE UBCH5B-CNOT4 COMPLEX
Overview
The protein CNOT4 possesses an N-terminal RING finger domain that acts as an E3 ubiquitin ligase and specifically interacts with UbcH5B, a ubiquitin-conjugating enzyme. The structure of the CNOT4 RING domain has been solved and the amino acids important for the binding to UbcH5B have been mapped. Here, the residues of UbcH5B important for the binding to CNOT4 RING domain were identified by NMR chemical shift perturbation experiments, and these data were used to generate structural models of the complex with the program HADDOCK. Together with the NMR data, additional biochemical data were included in a second docking, and comparisons of the resulting model with the structure of the c-Cbl/UbcH7 complex reveal some significant differences, notably at specific residues, and give structural insights into the E2/E3 specificity.
About this Structure
1UR6 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural model of the UbcH5B/CNOT4 complex revealed by combining NMR, mutagenesis, and docking approaches., Dominguez C, Bonvin AM, Winkler GS, van Schaik FM, Timmers HT, Boelens R, Structure. 2004 Apr;12(4):633-44. PMID:15062086
Page seeded by OCA on Thu Mar 20 14:35:35 2008
Categories: Homo sapiens | Protein complex | Ubiquitin--protein ligase | Boelens, R. | Bonvin, A M.J J. | Dominguez, C. | Schaik, F M.A Van. | Timmers, H Th M. | Winkler, G S. | ZN | Ccr4-not complex | Ligase | Ring finger protein | Transcription regulation | Ubiquitin conjugating enzyme | Ubiquitin ligase
