1v4b

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[[Image:1v4b.gif|left|200px]]<br /><applet load="1v4b" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1v4b.gif|left|200px]]
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caption="1v4b, resolution 1.80&Aring;" />
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'''The crystal structure of AzoR (Azo Reductase) from Escherichia coli: Oxidized form'''<br />
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{{Structure
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|PDB= 1v4b |SIZE=350|CAPTION= <scene name='initialview01'>1v4b</scene>, resolution 1.80&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> and <scene name='pdbligand=IPA:ISOPROPYL ALCOHOL'>IPA</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Azobenzene_reductase Azobenzene reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.1.6 1.7.1.6]
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|GENE=
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}}
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'''The crystal structure of AzoR (Azo Reductase) from Escherichia coli: Oxidized form'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1V4B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=FMN:'>FMN</scene>, <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=IPA:'>IPA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Azobenzene_reductase Azobenzene reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.1.6 1.7.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V4B OCA].
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1V4B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V4B OCA].
==Reference==
==Reference==
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Three-dimensional structure of AzoR from Escherichia coli. An oxidereductase conserved in microorganisms., Ito K, Nakanishi M, Lee WC, Sasaki H, Zenno S, Saigo K, Kitade Y, Tanokura M, J Biol Chem. 2006 Jul 21;281(29):20567-76. Epub 2006 May 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16684776 16684776]
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Three-dimensional structure of AzoR from Escherichia coli. An oxidereductase conserved in microorganisms., Ito K, Nakanishi M, Lee WC, Sasaki H, Zenno S, Saigo K, Kitade Y, Tanokura M, J Biol Chem. 2006 Jul 21;281(29):20567-76. Epub 2006 May 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16684776 16684776]
[[Category: Azobenzene reductase]]
[[Category: Azobenzene reductase]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:31:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:40:41 2008''

Revision as of 12:40, 20 March 2008

Image:1v4b.gif


PDB ID 1v4b

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands: , and
Activity: Azobenzene reductase, with EC number 1.7.1.6
Coordinates: save as pdb, mmCIF, xml



The crystal structure of AzoR (Azo Reductase) from Escherichia coli: Oxidized form


Overview

The crystal structure of AzoR (azoreductase) has been determined in complex with FMN for two different crystal forms at 1.8 and 2.2 A resolution. AzoR is an oxidoreductase isolated from Escherichia coli as a protein responsible for the degradation of azo compounds. This enzyme is an FMN-dependent NADH-azoreductase and catalyzes the reductive cleavage of azo groups by a ping-pong mechanism. The structure suggests that AzoR acts in a homodimeric state forming the two identical catalytic sites to which both monomers contribute. The structure revealed that each monomer of AzoR has a flavodoxin-like structure, without the explicit overall amino acid sequence homology. Superposition of the structures from the two different crystal forms revealed the conformational change and suggested a mechanism for accommodating substrates of different size. Furthermore, comparison of the active site structure with that of NQO1 complexed with substrates provides clues to the possible substrate-binding mechanism of AzoR.

About this Structure

1V4B is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of AzoR from Escherichia coli. An oxidereductase conserved in microorganisms., Ito K, Nakanishi M, Lee WC, Sasaki H, Zenno S, Saigo K, Kitade Y, Tanokura M, J Biol Chem. 2006 Jul 21;281(29):20567-76. Epub 2006 May 9. PMID:16684776

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