1v5x
From Proteopedia
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| - | [[Image:1v5x.jpg|left|200px]] | + | [[Image:1v5x.jpg|left|200px]] |
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| - | '''Crystal structure of Phosphoribosyl anthranilate isomerase from Thermus Thermophilus''' | + | {{Structure |
| + | |PDB= 1v5x |SIZE=350|CAPTION= <scene name='initialview01'>1v5x</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Phosphoribosylanthranilate_isomerase Phosphoribosylanthranilate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.24 5.3.1.24] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of Phosphoribosyl anthranilate isomerase from Thermus Thermophilus''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1V5X is a [ | + | 1V5X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V5X OCA]. |
==Reference== | ==Reference== | ||
| - | Stabilization due to dimer formation of phosphoribosyl anthranilate isomerase from Thermus thermophilus HB8: X-ray Analysis and DSC experiments., Taka J, Ogasahara K, Jeyakanthan J, Kunishima N, Kuroishi C, Sugahara M, Yokoyama S, Yutani K, J Biochem. 2005 May;137(5):569-78. PMID:[http:// | + | Stabilization due to dimer formation of phosphoribosyl anthranilate isomerase from Thermus thermophilus HB8: X-ray Analysis and DSC experiments., Taka J, Ogasahara K, Jeyakanthan J, Kunishima N, Kuroishi C, Sugahara M, Yokoyama S, Yutani K, J Biochem. 2005 May;137(5):569-78. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15944409 15944409] |
[[Category: Phosphoribosylanthranilate isomerase]] | [[Category: Phosphoribosylanthranilate isomerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
[[Category: trpf]] | [[Category: trpf]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:41:15 2008'' |
Revision as of 12:41, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Activity: | Phosphoribosylanthranilate isomerase, with EC number 5.3.1.24 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Phosphoribosyl anthranilate isomerase from Thermus Thermophilus
Overview
The crystal structure of phosphoribosyl anthranilate isomerase (PRAI) from Thermus thermophilus HB8 (TtPRAI) was solved at 2.0 A resolution. The overall structure of TtPRAI with a dimeric structure was quite similar to that of PRAI from Thermotoga maritima (TmPRAI). In order to elucidate the stabilization mechanism of TtPRAI, its physicochemical properties were examined using DSC, CD, and analytical centrifugation at various pHs in relation to the association-dissociation of the subunits. Based on the experimental results for TtPRAI and the structural information on TtPRAI and TmPRAI, we found that: (i) the denaturation of TtPRAI at acidic pH is correlated with the dissociation of its dimeric form; (ii) the hydrophobic interaction of TtPRAI in the monomer structure is slightly greater than that of TmPRAI, but dimer interface of the TmPRAI is remarkably greater; (iii) the contributions of hydrogen bonds and ion bonds to the stability are similar to each other; and (iv) destabilization due to the presence of cavities in TtPRAI is greater than that of TmPRAI in both the monomer and dimer structures.
About this Structure
1V5X is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Stabilization due to dimer formation of phosphoribosyl anthranilate isomerase from Thermus thermophilus HB8: X-ray Analysis and DSC experiments., Taka J, Ogasahara K, Jeyakanthan J, Kunishima N, Kuroishi C, Sugahara M, Yokoyama S, Yutani K, J Biochem. 2005 May;137(5):569-78. PMID:15944409
Page seeded by OCA on Thu Mar 20 14:41:15 2008
Categories: Phosphoribosylanthranilate isomerase | Single protein | Thermus thermophilus | Kunishima, N. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Taka, J. | Yutani, K. | Alpha-beta barrel | Isomerase | Phosphoribosyl anthranilate isomerase | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic | Trpf
