1v7x
From Proteopedia
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| - | [[Image:1v7x.jpg|left|200px]] | + | [[Image:1v7x.jpg|left|200px]] |
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| - | '''Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate''' | + | {{Structure |
| + | |PDB= 1v7x |SIZE=350|CAPTION= <scene name='initialview01'>1v7x</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= chbp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=671 Vibrio proteolyticus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1V7X is a [ | + | 1V7X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_proteolyticus Vibrio proteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V7X OCA]. |
==Reference== | ==Reference== | ||
| - | Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold., Hidaka M, Honda Y, Kitaoka M, Nirasawa S, Hayashi K, Wakagi T, Shoun H, Fushinobu S, Structure. 2004 Jun;12(6):937-47. PMID:[http:// | + | Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold., Hidaka M, Honda Y, Kitaoka M, Nirasawa S, Hayashi K, Wakagi T, Shoun H, Fushinobu S, Structure. 2004 Jun;12(6):937-47. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15274915 15274915] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vibrio proteolyticus]] | [[Category: Vibrio proteolyticus]] | ||
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[[Category: beta-sandwich]] | [[Category: beta-sandwich]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:41:58 2008'' |
Revision as of 12:41, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Ligands: | , , and | ||||||
| Gene: | chbp (Vibrio proteolyticus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate
Overview
Vibrio proteolyticus chitobiose phosphorylase (ChBP) belongs to glycosyl transferase family 36 (GT-36), and catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. As the first known structures of a GT-36 enzyme, we determined the crystal structure of ChBP in a ternary complex with GlcNAc and SO(4). It is also the first structures of an inverting phosphorolytic enzyme in a complex with a sugar and a sulfate ion, and reveals a pseudo-ternary complex structure of enzyme-sugar-phosphate. ChBP comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain, constituting a distinctive structure among GT families. Instead, it shows significant structural similarity with glycoside hydrolase (GH) enzymes, glucoamylases (GH-15), and maltose phosphorylase (GH-65) in clan GH-L. The structural similarity reported here, together with distant sequence similarities between ChBP and GHs, led to the reclassification of family GT-36 into a novel GH family, namely GH-94.
About this Structure
1V7X is a Single protein structure of sequence from Vibrio proteolyticus. Full crystallographic information is available from OCA.
Reference
Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold., Hidaka M, Honda Y, Kitaoka M, Nirasawa S, Hayashi K, Wakagi T, Shoun H, Fushinobu S, Structure. 2004 Jun;12(6):937-47. PMID:15274915
Page seeded by OCA on Thu Mar 20 14:41:58 2008
Categories: Single protein | Vibrio proteolyticus | Fushinobu, S. | Hayashi, K. | Hidaka, M. | Honda, Y. | Kitaoka, M. | Nirasawa, S. | Shoun, H. | Wakagi, T. | CA | NAG | NDG | SO4 | (alpha/alpha)6 barrel | Beta-sandwich
