1vdh
From Proteopedia
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| - | [[Image:1vdh.gif|left|200px]] | + | [[Image:1vdh.gif|left|200px]] |
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| - | '''Structure-based functional identification of a novel heme-binding protein from thermus thermophilus HB8''' | + | {{Structure |
| + | |PDB= 1vdh |SIZE=350|CAPTION= <scene name='initialview01'>1vdh</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Structure-based functional identification of a novel heme-binding protein from thermus thermophilus HB8''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1VDH is a [ | + | 1VDH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VDH OCA]. |
==Reference== | ==Reference== | ||
| - | Structure-based functional identification of a novel heme-binding protein from Thermus thermophilus HB8., Ebihara A, Okamoto A, Kousumi Y, Yamamoto H, Masui R, Ueyama N, Yokoyama S, Kuramitsu S, J Struct Funct Genomics. 2005;6(1):21-32. PMID:[http:// | + | Structure-based functional identification of a novel heme-binding protein from Thermus thermophilus HB8., Ebihara A, Okamoto A, Kousumi Y, Yamamoto H, Masui R, Ueyama N, Yokoyama S, Kuramitsu S, J Struct Funct Genomics. 2005;6(1):21-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15965735 15965735] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
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[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:44:07 2008'' |
Revision as of 12:44, 20 March 2008
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Structure-based functional identification of a novel heme-binding protein from thermus thermophilus HB8
Overview
The TT1485 gene from Thermus thermophilus HB8 encodes a hypothetical protein of unknown function with about 20 sequence homologs of bacterial or archaeal origin. Together they form a family of uncharacterized proteins, the cluster of orthologous group COG3253. Using a combination of amino acid sequence analysis, three-dimensional structural studies and biochemical assays, we identified TT1485 as a novel heme-binding protein. The crystal structure reveals that this protein is a pentamer and each monomer exhibits a beta-barrel fold. TT1485 is structurally similar to muconolactone isomerase, but this provided no functional clues. Amino acid sequence analysis revealed remote homology to a heme enzyme, chlorite dismutase. Strikingly, amino acid residues that are highly conserved in the homologous hypothetical proteins and chlorite dismutase cluster around a deep cavity on the surface of each monomer. Molecular modeling shows that the cavity can accommodate a heme group with a strictly conserved His as a heme ligand. TT1485 reconstituted with iron protoporphyrin IX chloride gave a low chlorite dismutase activity, indicating that TT1485 catalyzes a reaction other than chlorite degradation. The presence of a possible Fe-His-Asp triad in the heme proximal site suggests that TT1485 functions as a novel heme peroxidase to detoxify hydrogen peroxide within the cell.
About this Structure
1VDH is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Structure-based functional identification of a novel heme-binding protein from Thermus thermophilus HB8., Ebihara A, Okamoto A, Kousumi Y, Yamamoto H, Masui R, Ueyama N, Yokoyama S, Kuramitsu S, J Struct Funct Genomics. 2005;6(1):21-32. PMID:15965735
Page seeded by OCA on Thu Mar 20 14:44:07 2008
Categories: Protein complex | Thermus thermophilus | Ebihara, A. | Inoue, Y. | Kousumi, Y. | Kuramitsu, S. | Masui, R. | Okamoto, A. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Shibata, T. | Ueyama, N. | Yamamoto, H. | Yokoyama, S. | Beta barrel | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic
