1vff
From Proteopedia
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| - | [[Image:1vff.gif|left|200px]] | + | [[Image:1vff.gif|left|200px]] |
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| - | '''beta-glycosidase from Pyrococcus horikoshii''' | + | {{Structure |
| + | |PDB= 1vff |SIZE=350|CAPTION= <scene name='initialview01'>1vff</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] | ||
| + | |GENE= PH0366 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii]) | ||
| + | }} | ||
| + | |||
| + | '''beta-glycosidase from Pyrococcus horikoshii''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1VFF is a [ | + | 1VFF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VFF OCA]. |
==Reference== | ==Reference== | ||
| - | X-ray structure of a membrane-bound beta-glycosidase from the hyperthermophilic archaeon Pyrococcus horikoshii., Akiba T, Nishio M, Matsui I, Harata K, Proteins. 2004 Nov 1;57(2):422-31. PMID:[http:// | + | X-ray structure of a membrane-bound beta-glycosidase from the hyperthermophilic archaeon Pyrococcus horikoshii., Akiba T, Nishio M, Matsui I, Harata K, Proteins. 2004 Nov 1;57(2):422-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15340929 15340929] |
[[Category: Beta-glucosidase]] | [[Category: Beta-glucosidase]] | ||
[[Category: Pyrococcus horikoshii]] | [[Category: Pyrococcus horikoshii]] | ||
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[[Category: Matsui, I.]] | [[Category: Matsui, I.]] | ||
[[Category: Nishio, M.]] | [[Category: Nishio, M.]] | ||
| - | [[Category: | + | [[Category: alkylglucoside]] |
[[Category: glycosyl hydrolase]] | [[Category: glycosyl hydrolase]] | ||
[[Category: membrane-bound enzyme]] | [[Category: membrane-bound enzyme]] | ||
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[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:44:49 2008'' |
Revision as of 12:44, 20 March 2008
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| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | PH0366 (Pyrococcus horikoshii) | ||||||
| Activity: | Beta-glucosidase, with EC number 3.2.1.21 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
beta-glycosidase from Pyrococcus horikoshii
Overview
The beta-glycosidase of the hyperthermophilic Archaeon Pyrococcus horikoshii is a membrane-bound enzyme with the preferred substrate of alkyl-beta-glycosides. In this study, the unusual structural features that confer the extreme thermostability and substrate preferences of this enzyme were investigated by X-ray crystallography and docking simulation. The enzyme was crystallized in the presence of a neutral surfactant, and the crystal structure was solved by the molecular replacement method and refined at 2.5 A. The main-chain fold of the enzyme belongs to the (betaalpha)8 barrel structure common to the Family 1 glycosyl hydrolases. The active site is located at the center of the C-termini of the barrel beta-strands. The deep pocket of the active site accepts one sugar unit, and a hydrophobic channel extending radially from there binds the nonsugar moiety of the substrate. The docking simulation for oligosaccharides and alkylglucosides indicated that alkylglucosides with a long aliphatic chain are easily accommodated in the hydrophobic channel. This sparingly soluble enzyme has a cluster of hydrophobic residues on its surface, situated at the distal end of the active site channel and surrounded by a large patch of positively charged residues. We propose that this hydrophobic region can be inserted into the membrane while the surrounding positively charged residues make favorable contacts with phosphate groups on the inner surface of the membrane. The enzyme could thus adhere to the membrane in the proximity of its glycolipid substrate.
About this Structure
1VFF is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.
Reference
X-ray structure of a membrane-bound beta-glycosidase from the hyperthermophilic archaeon Pyrococcus horikoshii., Akiba T, Nishio M, Matsui I, Harata K, Proteins. 2004 Nov 1;57(2):422-31. PMID:15340929
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