1w0j

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[[Image:1w0j.gif|left|200px]]<br /><applet load="1w0j" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1w0j.gif|left|200px]]
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caption="1w0j, resolution 2.20&Aring;" />
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'''BERYLLIUM FLUORIDE INHIBITED BOVINE F1-ATPASE'''<br />
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{{Structure
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|PDB= 1w0j |SIZE=350|CAPTION= <scene name='initialview01'>1w0j</scene>, resolution 2.20&Aring;
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|SITE= <scene name='pdbsite=PLP:Bef+Binding+Site+For+Chain+F'>PLP</scene>
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14]
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|GENE=
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}}
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'''BERYLLIUM FLUORIDE INHIBITED BOVINE F1-ATPASE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1W0J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ADP:'>ADP</scene>, <scene name='pdbligand=BEF:'>BEF</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] Known structural/functional Site: <scene name='pdbsite=PLP:Bef+Binding+Site+For+Chain+F'>PLP</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0J OCA].
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1W0J is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0J OCA].
==Reference==
==Reference==
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The structure of bovine F1-ATPase inhibited by ADP and beryllium fluoride., Kagawa R, Montgomery MG, Braig K, Leslie AG, Walker JE, EMBO J. 2004 Jul 21;23(14):2734-44. Epub 2004 Jul 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15229653 15229653]
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The structure of bovine F1-ATPase inhibited by ADP and beryllium fluoride., Kagawa R, Montgomery MG, Braig K, Leslie AG, Walker JE, EMBO J. 2004 Jul 21;23(14):2734-44. Epub 2004 Jul 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15229653 15229653]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: H(+)-transporting two-sector ATPase]]
[[Category: H(+)-transporting two-sector ATPase]]
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[[Category: hydrolase]]
[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:39:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:50:28 2008''

Revision as of 12:50, 20 March 2008

Image:1w0j.gif


PDB ID 1w0j

Drag the structure with the mouse to rotate
, resolution 2.20Å
Sites:
Ligands: , , , and
Activity: H(+)-transporting two-sector ATPase, with EC number 3.6.3.14
Coordinates: save as pdb, mmCIF, xml



BERYLLIUM FLUORIDE INHIBITED BOVINE F1-ATPASE


Overview

The structure of bovine F1-ATPase inhibited with ADP and beryllium fluoride at 2.0 angstroms resolution contains two ADP.BeF3- complexes mimicking ATP, bound in the catalytic sites of the beta(TP) and beta(DP) subunits. Except for a 1 angstrom shift in the guanidinium of alphaArg373, the conformations of catalytic side chains are very similar in both sites. However, the ordered water molecule that carries out nucleophilic attack on the gamma-phosphate of ATP during hydrolysis is 2.6 angstroms from the beryllium in the beta(DP) subunit and 3.8 angstroms away in the beta(TP) subunit, strongly indicating that the beta(DP) subunit is the catalytically active conformation. In the structure of F1-ATPase with five bound ADP molecules (three in alpha-subunits, one each in the beta(TP) and beta(DP) subunits), which has also been determined, the conformation of alphaArg373 suggests that it senses the presence (or absence) of the gamma-phosphate of ATP. Two catalytic schemes are discussed concerning the various structures of bovine F1-ATPase.

About this Structure

1W0J is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

The structure of bovine F1-ATPase inhibited by ADP and beryllium fluoride., Kagawa R, Montgomery MG, Braig K, Leslie AG, Walker JE, EMBO J. 2004 Jul 21;23(14):2734-44. Epub 2004 Jul 1. PMID:15229653

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