3rr5
From Proteopedia
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| - | + | ==DNA ligase from the archaeon Thermococcus sp. 1519== | |
| - | === | + | <StructureSection load='3rr5' size='340' side='right' caption='[[3rr5]], [[Resolution|resolution]] 3.02Å' scene=''> |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3rr5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_sp._1519 Thermococcus sp. 1519]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RR5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RR5 FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2cfm|2cfm]], [[3gde|3gde]], [[2hiv|2hiv]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lig ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=619742 Thermococcus sp. 1519])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rr5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rr5 RCSB], [http://www.ebi.ac.uk/pdbsum/3rr5 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/C0LJI8_9EURY C0LJI8_9EURY]] DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair (By similarity).[HAMAP-Rule:MF_00407] | [[http://www.uniprot.org/uniprot/C0LJI8_9EURY C0LJI8_9EURY]] DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair (By similarity).[HAMAP-Rule:MF_00407] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA ligases join single-strand breaks in double-stranded DNA by catalyzing the formation of a phosphodiester bond between adjacent 5'-phosphate and 3'-hydroxyl termini. Their function is essential for maintaining genome integrity in the replication, recombination and repair of DNA. High flexibility is important for the function of DNA ligase molecules. Two types of overall conformations of archaeal DNA ligase that depend on the relative position of the OB-fold domain have previously been revealed: closed and open extended conformations. The structure of ATP-dependent DNA ligase from Thermococcus sp. 1519 (LigTh1519) in the crystalline state determined at a resolution of 3.02 A shows a new relative arrangement of the OB-fold domain which is intermediate between the positions of this domain in the closed and the open extended conformations of previously determined archaeal DNA ligases. However, small-angle X-ray scattering (SAXS) measurements indicate that in solution the LigTh1519 molecule adopts either an open extended conformation or both an intermediate and an open extended conformation with the open extended conformation being dominant. | ||
| + | |||
| + | ATP-dependent DNA ligase from Thermococcus sp. 1519 displays a new arrangement of the OB-fold domain.,Petrova T, Bezsudnova EY, Boyko KM, Mardanov AV, Polyakov KM, Volkov VV, Kozin M, Ravin NV, Shabalin IG, Skryabin KG, Stekhanova TN, Kovalchuk MV, Popov VO Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1440-7., doi: 10.1107/S1744309112043394. Epub 2012 Nov 14. PMID:23192021<ref>PMID:23192021</ref> | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
==See Also== | ==See Also== | ||
*[[DNA ligase|DNA ligase]] | *[[DNA ligase|DNA ligase]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Thermococcus sp. 1519]] | [[Category: Thermococcus sp. 1519]] | ||
| - | [[Category: Bezsudnova, E Y | + | [[Category: Bezsudnova, E Y]] |
| - | [[Category: Boyko, K M | + | [[Category: Boyko, K M]] |
| - | [[Category: Kovalchuk, M V | + | [[Category: Kovalchuk, M V]] |
| - | [[Category: Mardanov, A V | + | [[Category: Mardanov, A V]] |
| - | [[Category: Petrova, T | + | [[Category: Petrova, T]] |
| - | [[Category: Polyakov, K M | + | [[Category: Polyakov, K M]] |
| - | [[Category: Popov, V O | + | [[Category: Popov, V O]] |
| - | [[Category: Ravin, N V | + | [[Category: Ravin, N V]] |
| - | [[Category: Shabalin, I G | + | [[Category: Shabalin, I G]] |
| - | [[Category: Skryabin, K G | + | [[Category: Skryabin, K G]] |
| - | [[Category: Stekhanova, T N | + | [[Category: Stekhanova, T N]] |
[[Category: Archaeon]] | [[Category: Archaeon]] | ||
[[Category: Atp-dependent thermostable dna ligase]] | [[Category: Atp-dependent thermostable dna ligase]] | ||
[[Category: Ligase]] | [[Category: Ligase]] | ||
Revision as of 08:35, 24 December 2014
DNA ligase from the archaeon Thermococcus sp. 1519
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