1waf
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1waf.gif|left|200px]] | + | [[Image:1waf.gif|left|200px]] |
| - | + | ||
| - | '''DNA POLYMERASE FROM BACTERIOPHAGE RB69''' | + | {{Structure |
| + | |PDB= 1waf |SIZE=350|CAPTION= <scene name='initialview01'>1waf</scene>, resolution 3.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=GMP:GUANOSINE'>GMP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] | ||
| + | |GENE= POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10692 Enterobacteria phage RB18]) | ||
| + | }} | ||
| + | |||
| + | '''DNA POLYMERASE FROM BACTERIOPHAGE RB69''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1WAF is a [ | + | 1WAF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb18 Enterobacteria phage rb18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAF OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69., Wang J, Sattar AK, Wang CC, Karam JD, Konigsberg WH, Steitz TA, Cell. 1997 Jun 27;89(7):1087-99. PMID:[http:// | + | Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69., Wang J, Sattar AK, Wang CC, Karam JD, Konigsberg WH, Steitz TA, Cell. 1997 Jun 27;89(7):1087-99. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9215631 9215631] |
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
[[Category: Enterobacteria phage rb18]] | [[Category: Enterobacteria phage rb18]] | ||
| Line 24: | Line 33: | ||
[[Category: rb69 dna polymerase (gp43)]] | [[Category: rb69 dna polymerase (gp43)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:54:23 2008'' |
Revision as of 12:54, 20 March 2008
| |||||||
| , resolution 3.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | POL (Enterobacteria phage RB18) | ||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DNA POLYMERASE FROM BACTERIOPHAGE RB69
Overview
The 2.8 A resolution crystal structure of the bacteriophage RB69 gp43, a member of the eukaryotic pol alpha family of replicative DNA polymerases, shares some similarities with other polymerases but shows many differences. Although its palm domain has the same topology as other polymerases, except rat DNA polymerase beta, one of the three carboxylates required for nucleotidyl transfer is located on a different beta strand. The structures of the fingers and thumb domains are unrelated to all other known polymerase structures. The editing 3'-5' exonuclease domain of gp43 is homologous to that of E. coli DNA polymerase I but lies on the opposite side of the polymerase active site. An extended structure-based alignment of eukaryotic DNA polymerase sequences provides structural insights that should be applicable to most eukaryotic DNA polymerases.
About this Structure
1WAF is a Single protein structure of sequence from Enterobacteria phage rb18. Full crystallographic information is available from OCA.
Reference
Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69., Wang J, Sattar AK, Wang CC, Karam JD, Konigsberg WH, Steitz TA, Cell. 1997 Jun 27;89(7):1087-99. PMID:9215631
Page seeded by OCA on Thu Mar 20 14:54:23 2008
