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1wac

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[[Image:1wac.gif|left|200px]]<br /><applet load="1wac" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1wac.gif|left|200px]]
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caption="1wac, resolution 3.0&Aring;" />
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'''BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE'''<br />
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{{Structure
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|PDB= 1wac |SIZE=350|CAPTION= <scene name='initialview01'>1wac</scene>, resolution 3.0&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1WAC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_phi-6 Bacteriophage phi-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA].
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1WAC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_phi-6 Bacteriophage phi-6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA].
==Reference==
==Reference==
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Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15659773 15659773]
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Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15659773 15659773]
[[Category: Bacteriophage phi-6]]
[[Category: Bacteriophage phi-6]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: transcription]]
[[Category: transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:42:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:54:22 2008''

Revision as of 12:54, 20 March 2008

Image:1wac.gif


PDB ID 1wac

Drag the structure with the mouse to rotate
, resolution 3.0Å
Coordinates: save as pdb, mmCIF, xml



BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE


Overview

The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.

About this Structure

1WAC is a Single protein structure of sequence from Bacteriophage phi-6. Full crystallographic information is available from OCA.

Reference

Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:15659773

Page seeded by OCA on Thu Mar 20 14:54:22 2008

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