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1wco
From Proteopedia
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| - | [[Image:1wco.gif|left|200px]]< | + | [[Image:1wco.gif|left|200px]] |
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| - | '''THE SOLUTION STRUCTURE OF THE NISIN-LIPID II COMPLEX''' | + | {{Structure |
| + | |PDB= 1wco |SIZE=350|CAPTION= <scene name='initialview01'>1wco</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=MUB:N-ACETYLMURAMIC+ACID'>MUB</scene> and <scene name='pdbligand=FPP:FARNESYL DIPHOSPHATE'>FPP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THE SOLUTION STRUCTURE OF THE NISIN-LIPID II COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1WCO is a [ | + | 1WCO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis] and [http://en.wikipedia.org/wiki/Monarthropalpus_flavus Monarthropalpus flavus]. This structure supersedes the now removed PDB entry 1UZT. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WCO OCA]. |
==Reference== | ==Reference== | ||
| - | The nisin-lipid II complex reveals a pyrophosphate cage that provides a blueprint for novel antibiotics., Hsu ST, Breukink E, Tischenko E, Lutters MA, de Kruijff B, Kaptein R, Bonvin AM, van Nuland NA, Nat Struct Mol Biol. 2004 Oct;11(10):963-7. Epub 2004 Sep 12. PMID:[http:// | + | The nisin-lipid II complex reveals a pyrophosphate cage that provides a blueprint for novel antibiotics., Hsu ST, Breukink E, Tischenko E, Lutters MA, de Kruijff B, Kaptein R, Bonvin AM, van Nuland NA, Nat Struct Mol Biol. 2004 Oct;11(10):963-7. Epub 2004 Sep 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15361862 15361862] |
[[Category: Lactococcus lactis]] | [[Category: Lactococcus lactis]] | ||
[[Category: Monarthropalpus flavus]] | [[Category: Monarthropalpus flavus]] | ||
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[[Category: MUB]] | [[Category: MUB]] | ||
[[Category: NAG]] | [[Category: NAG]] | ||
| - | [[Category: | + | [[Category: antibiotic]] |
[[Category: peptidoglycan]] | [[Category: peptidoglycan]] | ||
[[Category: pore formation]] | [[Category: pore formation]] | ||
[[Category: pyrophosphate cage]] | [[Category: pyrophosphate cage]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:55:16 2008'' |
Revision as of 12:55, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE SOLUTION STRUCTURE OF THE NISIN-LIPID II COMPLEX
Overview
The emerging antibiotics-resistance problem has underlined the urgent need for novel antimicrobial agents. Lantibiotics (lanthionine-containing antibiotics) are promising candidates to alleviate this problem. Nisin, a member of this family, has a unique pore-forming activity against bacteria. It binds to lipid II, the essential precursor of cell wall synthesis. As a result, the membrane permeabilization activity of nisin is increased by three orders of magnitude. Here we report the solution structure of the complex of nisin and lipid II. The structure shows a novel lipid II-binding motif in which the pyrophosphate moiety of lipid II is primarily coordinated by the N-terminal backbone amides of nisin via intermolecular hydrogen bonds. This cage structure provides a rationale for the conservation of the lanthionine rings among several lipid II-binding lantibiotics. The structure of the pyrophosphate cage offers a template for structure-based design of novel antibiotics.
About this Structure
1WCO is a Single protein structure of sequence from Lactococcus lactis and Monarthropalpus flavus. This structure supersedes the now removed PDB entry 1UZT. Full crystallographic information is available from OCA.
Reference
The nisin-lipid II complex reveals a pyrophosphate cage that provides a blueprint for novel antibiotics., Hsu ST, Breukink E, Tischenko E, Lutters MA, de Kruijff B, Kaptein R, Bonvin AM, van Nuland NA, Nat Struct Mol Biol. 2004 Oct;11(10):963-7. Epub 2004 Sep 12. PMID:15361862
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