3nla
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Antifreeze proteins (AFPs) have the unique ability to adsorb to ice and, inhibit its growth. Many organisms ranging from fish to bacteria use AFPs, to retard freezing or lessen the damage incurred upon freezing and, thawing. The ice-binding mechanism of the long linear alpha-helical type I, AFPs has been attributed to their regularly spaced polar residues matching, the ice lattice along a pyramidal plane. In contrast, it is not known how, globular antifreeze proteins such as type III AFP that lack repeating, ice-binding residues bind to ice. Here we report the 1.25 A crystal, structure of recombinant type III AFP (QAE isoform) from eel pout, (Macrozoarces americanus), which reveals a remarkably flat amphipathic, ice-binding site where five hydrogen-bonding atoms match two ranks of, | + | Antifreeze proteins (AFPs) have the unique ability to adsorb to ice and, inhibit its growth. Many organisms ranging from fish to bacteria use AFPs, to retard freezing or lessen the damage incurred upon freezing and, thawing. The ice-binding mechanism of the long linear alpha-helical type I, AFPs has been attributed to their regularly spaced polar residues matching, the ice lattice along a pyramidal plane. In contrast, it is not known how, globular antifreeze proteins such as type III AFP that lack repeating, ice-binding residues bind to ice. Here we report the 1.25 A crystal, structure of recombinant type III AFP (QAE isoform) from eel pout, (Macrozoarces americanus), which reveals a remarkably flat amphipathic, ice-binding site where five hydrogen-bonding atoms match two ranks of, oxygens on the [1010] ice prism plane in the <0001> direction, giving high, ice-binding affinity and specificity. This binding site, substantiated by, the structures and properties of several ice-binding site mutants, suggests that the AFP occupies a niche in the ice surface in which it, covers the basal plane while binding to the prism face. |
==About this Structure== | ==About this Structure== | ||
| - | 3NLA is a | + | 3NLA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lycodichthys_dearborni Lycodichthys dearborni]. Structure known Active Site: 1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3NLA OCA]. |
==Reference== | ==Reference== | ||
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[[Category: thermal hysteresis protein]] | [[Category: thermal hysteresis protein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 13:02:08 2007'' |
Revision as of 10:56, 5 November 2007
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NMR STRUCTURE OF THE N-TERMINAL DOMAIN WITH A LINKER PORTION OF ANTARCTIC EEL POUT ANTIFREEZE PROTEIN RD3, 40 STRUCTURES
Overview
Antifreeze proteins (AFPs) have the unique ability to adsorb to ice and, inhibit its growth. Many organisms ranging from fish to bacteria use AFPs, to retard freezing or lessen the damage incurred upon freezing and, thawing. The ice-binding mechanism of the long linear alpha-helical type I, AFPs has been attributed to their regularly spaced polar residues matching, the ice lattice along a pyramidal plane. In contrast, it is not known how, globular antifreeze proteins such as type III AFP that lack repeating, ice-binding residues bind to ice. Here we report the 1.25 A crystal, structure of recombinant type III AFP (QAE isoform) from eel pout, (Macrozoarces americanus), which reveals a remarkably flat amphipathic, ice-binding site where five hydrogen-bonding atoms match two ranks of, oxygens on the [1010] ice prism plane in the <0001> direction, giving high, ice-binding affinity and specificity. This binding site, substantiated by, the structures and properties of several ice-binding site mutants, suggests that the AFP occupies a niche in the ice surface in which it, covers the basal plane while binding to the prism face.
About this Structure
3NLA is a Single protein structure of sequence from Lycodichthys dearborni. Structure known Active Site: 1. Full crystallographic information is available from OCA.
Reference
Structural basis for the binding of a globular antifreeze protein to ice., Jia Z, DeLuca CI, Chao H, Davies PL, Nature. 1996 Nov 21;384(6606):285-8. PMID:8918883
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