4wf0
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Crystal Structure of iLID - an Improved Light-Inducible Dimer== |
| + | <StructureSection load='4wf0' size='340' side='right' caption='[[4wf0]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4wf0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WF0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WF0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wf0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wf0 RCSB], [http://www.ebi.ac.uk/pdbsum/4wf0 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Photocontrol of functional peptides is a powerful tool for spatial and temporal control of cell signaling events. We show that the genetically encoded light-sensitive LOV2 domain of Avena Sativa phototropin 1 (AsLOV2) can be used to reversibly photomodulate the affinity of peptides for their binding partners. Sequence analysis and molecular modeling were used to embed two peptides into the Jalpha helix of the AsLOV2 domain while maintaining AsLOV2 structure in the dark but allowing for binding to effector proteins when the Jalpha helix unfolds in the light. Caged versions of the ipaA and SsrA peptides, LOV-ipaA and LOV-SsrA, bind their targets with 49- and 8-fold enhanced affinity in the light, respectively. These switches can be used as general tools for light-dependent colocalization, which we demonstrate with photo-activable gene transcription in yeast. | ||
| - | + | Designing photoswitchable peptides using the AsLOV2 domain.,Lungu OI, Hallett RA, Choi EJ, Aiken MJ, Hahn KM, Kuhlman B Chem Biol. 2012 Apr 20;19(4):507-17. doi: 10.1016/j.chembiol.2012.02.006. PMID:22520757<ref>PMID:22520757</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Hallett, R]] | ||
| + | [[Category: Kuhlman, B]] | ||
| + | [[Category: Williams, T]] | ||
| + | [[Category: Flavoprotein]] | ||
| + | [[Category: Light-inducible dimer]] | ||
Revision as of 10:51, 24 December 2014
Crystal Structure of iLID - an Improved Light-Inducible Dimer
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