4gyh

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[[Image:4gyh.jpg|left|200px]]
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==Structure of human thymidylate synthase at high salt conditions==
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<StructureSection load='4gyh' size='340' side='right' caption='[[4gyh]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4gyh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GYH FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4h1i|4h1i]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TYMS, TS, OK/SW-cl.29 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gyh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gyh RCSB], [http://www.ebi.ac.uk/pdbsum/4gyh PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/TYSY_HUMAN TYSY_HUMAN]] Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.<ref>PMID:21876188</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Expression of hTS (human thymidylate synthase), a key enzyme in thymidine biosynthesis, is regulated on the translational level through a feedback mechanism that is rarely found in eukaryotes. At low substrate concentrations, the ligand-free enzyme binds to its own mRNA and stabilizes a hairpin structure that sequesters the start codon. When in complex with dUMP (2'-deoxyuridine-5'-monophosphate) and a THF (tetrahydrofolate) cofactor, the enzyme adopts a conformation that is unable to bind and repress expression of mRNA. Here, we have used a combination of X-ray crystallography, RNA mutagenesis and site-specific cross-linking studies to investigate the molecular recognition of TS mRNA by the hTS enzyme. The interacting mRNA region was narrowed to the start codon and immediately flanking sequences. In the hTS enzyme, a helix-loop-helix domain on the protein surface was identified as the putative RNA-binding site.
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{{STRUCTURE_4gyh| PDB=4gyh | SCENE= }}
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Analysis of mRNA recognition by human thymidylate synthase.,Brunn ND, Dibrov SM, Kao MB, Ghassemian M, Hermann T Biosci Rep. 2014 Dec 23;34(6). pii: e00168. doi: 10.1042/BSR20140137. PMID:25423174<ref>PMID:25423174</ref>
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===Structure of human thymidylate synthase at high salt conditions===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
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==About this Structure==
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*[[Thymidylate synthase|Thymidylate synthase]]
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[[4gyh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GYH OCA].
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== References ==
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[[Category: Homo sapiens]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Human]]
[[Category: Thymidylate synthase]]
[[Category: Thymidylate synthase]]
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[[Category: Brunn, N.]]
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[[Category: Brunn, N]]
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[[Category: Dibrov, S.]]
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[[Category: Dibrov, S]]
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[[Category: Hermann, T.]]
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[[Category: Hermann, T]]
[[Category: Methyl transferase]]
[[Category: Methyl transferase]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 10:56, 24 December 2014

Structure of human thymidylate synthase at high salt conditions

4gyh, resolution 3.00Å

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