1wl7
From Proteopedia
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| - | [[Image:1wl7.gif|left|200px]] | + | [[Image:1wl7.gif|left|200px]] |
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| - | '''Structure of the thermostable arabinanase''' | + | {{Structure |
| + | |PDB= 1wl7 |SIZE=350|CAPTION= <scene name='initialview01'>1wl7</scene>, resolution 1.90Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Arabinan_endo-1,5-alpha-L-arabinosidase Arabinan endo-1,5-alpha-L-arabinosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.99 3.2.1.99] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structure of the thermostable arabinanase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1WL7 is a [ | + | 1WL7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_thermodenitrificans Geobacillus thermodenitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WL7 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for thermostability of endo-1,5-alpha-L-arabinanase from Bacillus thermodenitrificans TS-3., Yamaguchi A, Tada T, Wada K, Nakaniwa T, Kitatani T, Sogabe Y, Takao M, Sakai T, Nishimura K, J Biochem. 2005 May;137(5):587-92. PMID:[http:// | + | Structural basis for thermostability of endo-1,5-alpha-L-arabinanase from Bacillus thermodenitrificans TS-3., Yamaguchi A, Tada T, Wada K, Nakaniwa T, Kitatani T, Sogabe Y, Takao M, Sakai T, Nishimura K, J Biochem. 2005 May;137(5):587-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15944411 15944411] |
[[Category: Arabinan endo-1,5-alpha-L-arabinosidase]] | [[Category: Arabinan endo-1,5-alpha-L-arabinosidase]] | ||
[[Category: Geobacillus thermodenitrificans]] | [[Category: Geobacillus thermodenitrificans]] | ||
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[[Category: Yamaguchi, A.]] | [[Category: Yamaguchi, A.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
| - | [[Category: abn- | + | [[Category: abn-t]] |
[[Category: arabinanase]] | [[Category: arabinanase]] | ||
[[Category: bacillus]] | [[Category: bacillus]] | ||
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[[Category: thermostable enzyme]] | [[Category: thermostable enzyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:58:22 2008'' |
Revision as of 12:58, 20 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Arabinan endo-1,5-alpha-L-arabinosidase, with EC number 3.2.1.99 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the thermostable arabinanase
Overview
The crystal structure of a thermostable endo-1,5-alpha-L-arabinanase, ABN-TS, from Bacillus thermodenitrificans TS-3 was determined at 1.9 A to an R-factor of 18.3% and an R-free-factor of 22.5%. The enzyme molecule has a five-bladed beta-propeller fold. The substrate-binding cleft formed across one face of the propeller is open on both sides to allow random binding of several sugar units in the polymeric substrate arabinan. The beta-propeller fold is stabilized through a ring closure. ABN-TS exhibits a new closure-mode involving residues in the N-terminal region: Phe7 to Gly21 exhibit hydrogen bonds and hydrophobic interactions with the first and last blades, and Phe4 links the second and third blades through a hydrogen bond and an aromatic stacking interaction, respectively. The role of the N-terminal region in the thermostability was confirmed with a mutant lacking 16 amino acid residues from the N-terminus of ABN-TS.
About this Structure
1WL7 is a Single protein structure of sequence from Geobacillus thermodenitrificans. Full crystallographic information is available from OCA.
Reference
Structural basis for thermostability of endo-1,5-alpha-L-arabinanase from Bacillus thermodenitrificans TS-3., Yamaguchi A, Tada T, Wada K, Nakaniwa T, Kitatani T, Sogabe Y, Takao M, Sakai T, Nishimura K, J Biochem. 2005 May;137(5):587-92. PMID:15944411
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