1wr1
From Proteopedia
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| - | [[Image:1wr1.gif|left|200px]] | + | [[Image:1wr1.gif|left|200px]] |
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| - | '''The complex sturcture of Dsk2p UBA with ubiquitin''' | + | {{Structure |
| + | |PDB= 1wr1 |SIZE=350|CAPTION= <scene name='initialview01'>1wr1</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= UBI4 (1224-1451) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), DSK2 (982-1120) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''The complex sturcture of Dsk2p UBA with ubiquitin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1WR1 is a [ | + | 1WR1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WR1 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition., Ohno A, Jee J, Fujiwara K, Tenno T, Goda N, Tochio H, Kobayashi H, Hiroaki H, Shirakawa M, Structure. 2005 Apr;13(4):521-32. PMID:[http:// | + | Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition., Ohno A, Jee J, Fujiwara K, Tenno T, Goda N, Tochio H, Kobayashi H, Hiroaki H, Shirakawa M, Structure. 2005 Apr;13(4):521-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15837191 15837191] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: uba-ubiquitin complex]] | [[Category: uba-ubiquitin complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:00:30 2008'' |
Revision as of 13:00, 20 March 2008
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| Gene: | UBI4 (1224-1451) (Saccharomyces cerevisiae), DSK2 (982-1120) (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The complex sturcture of Dsk2p UBA with ubiquitin
Overview
The ubiquitin-associated (UBA) domain is one of the most frequently occurring motifs that recognize ubiquitin tags. Dsk2p, a UBA-containing protein from Saccharomyces cerevisiae, is involved in the ubiquitin-proteasome proteolytic pathway and has been implicated in spindle pole duplication. Here we present the solution structure of the UBA domain of Dsk2p (Dsk2(UBA)) in complex with ubiquitin. The structure reveals that the UBA domain uses a mode of ubiquitin recognition that is similar to that of the CUE domain, another ubiquitin binding motif that shares low sequence homology but high structural similarity with UBA domains. These two domains, as well as the structurally unrelated ubiquitin binding motif UIM, provide a common, crucial recognition site for ubiquitin, comprising a hydrogen-bonding acceptor for the amide group of Gly-47, and a methyl group that packs against the hydrophobic pocket of ubiquitin formed by Leu-8, Ile-44, His-68, and Val-70.
About this Structure
1WR1 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition., Ohno A, Jee J, Fujiwara K, Tenno T, Goda N, Tochio H, Kobayashi H, Hiroaki H, Shirakawa M, Structure. 2005 Apr;13(4):521-32. PMID:15837191
Page seeded by OCA on Thu Mar 20 15:00:30 2008
