1x0g

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[[Image:1x0g.gif|left|200px]]<br /><applet load="1x0g" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1x0g.gif|left|200px]]
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caption="1x0g, resolution 2.50&Aring;" />
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'''Crystal Structure of IscA with the [2Fe-2S] cluster'''<br />
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{{Structure
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|PDB= 1x0g |SIZE=350|CAPTION= <scene name='initialview01'>1x0g</scene>, resolution 2.50&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=FES:FE2/S2 (INORGANIC) CLUSTER'>FES</scene>
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|ACTIVITY=
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|GENE= tll0464 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria])
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}}
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'''Crystal Structure of IscA with the [2Fe-2S] cluster'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1X0G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=FES:'>FES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X0G OCA].
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1X0G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X0G OCA].
==Reference==
==Reference==
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The asymmetric IscA homodimer with an exposed [2Fe-2S] cluster suggests the structural basis of the Fe-S cluster biosynthetic scaffold., Morimoto K, Yamashita E, Kondou Y, Lee SJ, Arisaka F, Tsukihara T, Nakai M, J Mol Biol. 2006 Jun 30;360(1):117-32. Epub 2006 May 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16730357 16730357]
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The asymmetric IscA homodimer with an exposed [2Fe-2S] cluster suggests the structural basis of the Fe-S cluster biosynthetic scaffold., Morimoto K, Yamashita E, Kondou Y, Lee SJ, Arisaka F, Tsukihara T, Nakai M, J Mol Biol. 2006 Jun 30;360(1):117-32. Epub 2006 May 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16730357 16730357]
[[Category: Bacteria]]
[[Category: Bacteria]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: sulfur]]
[[Category: sulfur]]
[[Category: tetrameric]]
[[Category: tetrameric]]
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[[Category: three conserved cys]]
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[[Category: three conserved cy]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:49:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:03:42 2008''

Revision as of 13:03, 20 March 2008

Image:1x0g.gif


PDB ID 1x0g

Drag the structure with the mouse to rotate
, resolution 2.50Å
Ligands: and
Gene: tll0464 (Bacteria)
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of IscA with the [2Fe-2S] cluster


Overview

It has been shown that the so-called scaffold proteins are vital in Fe-S cluster biosynthesis by providing an intermediate site for the assembly of Fe-S clusters. However, since no structural information on such scaffold proteins with bound Fe-S cluster intermediates is available, the structural basis of the core of Fe-S cluster biosynthesis remains poorly understood. Here we report the first Fe-S cluster-bound crystal structure of a scaffold protein, IscA, from Thermosynechococcus elongatus, which carries three strictly conserved cysteine residues. Surprisingly, one partially exposed [2Fe-2S] cluster is coordinated by two conformationally distinct IscA protomers, termed alpha and beta, with asymmetric cysteinyl ligation by Cys37, Cys101, Cys103 from alpha and Cys103 from beta. In the crystal, two alphabeta dimers form an unusual domain-swapped tetramer via central domains of beta protomers. Together with additional biochemical data supporting its physiologically relevant configuration, we propose that the unique asymmetric Fe-S cluster coordination and the resulting distinct conformational stabilities of the two IscA protomers are central to the function of IscA-type Fe-S cluster biosynthetic scaffold.

About this Structure

1X0G is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

The asymmetric IscA homodimer with an exposed [2Fe-2S] cluster suggests the structural basis of the Fe-S cluster biosynthetic scaffold., Morimoto K, Yamashita E, Kondou Y, Lee SJ, Arisaka F, Tsukihara T, Nakai M, J Mol Biol. 2006 Jun 30;360(1):117-32. Epub 2006 May 15. PMID:16730357[[Category: [2fe-2s]]]

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