1x8i

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[[Image:1x8i.jpg|left|200px]]<br /><applet load="1x8i" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1x8i.jpg|left|200px]]
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caption="1x8i, resolution 1.90&Aring;" />
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'''Crystal Structure of the Zinc Carbapenemase CphA in Complex with the Antibiotic Biapenem'''<br />
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{{Structure
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|PDB= 1x8i |SIZE=350|CAPTION= <scene name='initialview01'>1x8i</scene>, resolution 1.90&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=BMH:5H-PYRAZOLO(1,2-A)(1,2,4)TRIAZOL-4-IUM,+6-((2-CARBOXY-6-(1-HYDROXYETHYL)-4-METHYL-7-OXO-1-AZABICYCLO(3.2.0)HEPT-2-EN-3-YL)THIO)-6,7-DIHYDRO-,+HYDROXIDE,+INNER+SALT,+(4R-(4ALPHA,5BETA,6BETA(R*)))-'>BMH</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]
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|GENE= cphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=644 Aeromonas hydrophila])
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}}
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'''Crystal Structure of the Zinc Carbapenemase CphA in Complex with the Antibiotic Biapenem'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1X8I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=BMH:'>BMH</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8I OCA].
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1X8I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8I OCA].
==Reference==
==Reference==
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A metallo-beta-lactamase enzyme in action: crystal structures of the monozinc carbapenemase CphA and its complex with biapenem., Garau G, Bebrone C, Anne C, Galleni M, Frere JM, Dideberg O, J Mol Biol. 2005 Jan 28;345(4):785-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15588826 15588826]
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A metallo-beta-lactamase enzyme in action: crystal structures of the monozinc carbapenemase CphA and its complex with biapenem., Garau G, Bebrone C, Anne C, Galleni M, Frere JM, Dideberg O, J Mol Biol. 2005 Jan 28;345(4):785-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15588826 15588826]
[[Category: Aeromonas hydrophila]]
[[Category: Aeromonas hydrophila]]
[[Category: Beta-lactamase]]
[[Category: Beta-lactamase]]
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[[Category: hydrolase]]
[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:52:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:06:32 2008''

Revision as of 13:06, 20 March 2008

Image:1x8i.jpg


PDB ID 1x8i

Drag the structure with the mouse to rotate
, resolution 1.90Å
Ligands: , , and
Gene: cphA (Aeromonas hydrophila)
Activity: Beta-lactamase, with EC number 3.5.2.6
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of the Zinc Carbapenemase CphA in Complex with the Antibiotic Biapenem


Overview

One strategy developed by bacteria to resist the action of beta-lactam antibiotics is the expression of metallo-beta-lactamases. CphA from Aeromonas hydrophila is a member of a clinically important subclass of metallo-beta-lactamases that have only one zinc ion in their active site and for which no structure is available. The crystal structures of wild-type CphA and its N220G mutant show the structural features of the active site of this enzyme, which is modeled specifically for carbapenem hydrolysis. The structure of CphA after reaction with a carbapenem substrate, biapenem, reveals that the enzyme traps a reaction intermediate in the active site. These three X-ray structures have allowed us to propose how the enzyme recognizes carbapenems and suggest a mechanistic pathway for hydrolysis of the beta-lactam. This will be relevant for the design of metallo-beta-lactamase inhibitors as well as of antibiotics that escape their hydrolytic activity.

About this Structure

1X8I is a Single protein structure of sequence from Aeromonas hydrophila. Full crystallographic information is available from OCA.

Reference

A metallo-beta-lactamase enzyme in action: crystal structures of the monozinc carbapenemase CphA and its complex with biapenem., Garau G, Bebrone C, Anne C, Galleni M, Frere JM, Dideberg O, J Mol Biol. 2005 Jan 28;345(4):785-95. PMID:15588826

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