1xf2

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[[Image:1xf2.gif|left|200px]]<br /><applet load="1xf2" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1xf2.gif|left|200px]]
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caption="1xf2, resolution 2.30&Aring;" />
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'''Structure of Fab DNA-1 complexed with dT3'''<br />
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{{Structure
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|PDB= 1xf2 |SIZE=350|CAPTION= <scene name='initialview01'>1xf2</scene>, resolution 2.30&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Structure of Fab DNA-1 complexed with dT3'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1XF2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XF2 OCA].
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1XF2 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XF2 OCA].
==Reference==
==Reference==
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Evidence for structural plasticity of heavy chain complementarity-determining region 3 in antibody-ssDNA recognition., Schuermann JP, Prewitt SP, Davies C, Deutscher SL, Tanner JJ, J Mol Biol. 2005 Apr 15;347(5):965-78. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15784256 15784256]
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Evidence for structural plasticity of heavy chain complementarity-determining region 3 in antibody-ssDNA recognition., Schuermann JP, Prewitt SP, Davies C, Deutscher SL, Tanner JJ, J Mol Biol. 2005 Apr 15;347(5):965-78. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15784256 15784256]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: immunoglobulin]]
[[Category: immunoglobulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:54:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:08:56 2008''

Revision as of 13:08, 20 March 2008

Image:1xf2.gif


PDB ID 1xf2

Drag the structure with the mouse to rotate
, resolution 2.30Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



Structure of Fab DNA-1 complexed with dT3


Overview

Anti-DNA antibodies play important roles in the pathogenesis of autoimmune diseases. They also represent a unique and relatively unexplored class of DNA-binding protein. Here, we present a study of conformational changes induced by DNA binding to an anti-ssDNA Fab known as DNA-1. Three crystal structures are reported: a complex of DNA-1 bound to dT3, and two structures of the ligand-free Fab. One of the ligand-free structures was determined from crystals exhibiting perfect hemihedral twinning, and the details of structure determination are provided. Unexpectedly, five residues (H97-H100A) in the apex of heavy chain complementarity-determining region 3 (HCDR3) are disordered in both ligand-free structures. Ligand binding also caused a 2-4A shift of the backbone of Tyr L92 and ordering of the L92 side-chain. In contrast, these residues are highly ordered in the Fab/dT3 complex, where Tyr H100 and Tyr H100A form intimate stacking interactions with DNA bases, and L92 forms the 5' end of the binding site. The structures suggest that HCDR3 is very flexible and adopts multiple conformations in the ligand-free state. These results are discussed in terms of induced fit and pre-existing equilibrium theories of ligand binding. Our results allow new interpretations of existing thermodynamic and mutagenesis data in terms of conformational entropy and the volume of conformational space accessible to HCDR3 in the ligand-free state. In the context of autoimmune disease, plasticity of the ligand-free antibody could provide a mechanism by which anti-DNA antibodies bind diverse host ligands, and thereby contribute to pathogenicity.

About this Structure

1XF2 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Evidence for structural plasticity of heavy chain complementarity-determining region 3 in antibody-ssDNA recognition., Schuermann JP, Prewitt SP, Davies C, Deutscher SL, Tanner JJ, J Mol Biol. 2005 Apr 15;347(5):965-78. PMID:15784256

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