2ba0

From Proteopedia

(Difference between revisions)

Revision as of 15:09, 24 December 2014

Archaeal exosome core

Structural highlights

2ba0 is a 9 chain structure with sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2ba1
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[ECR1_ARCFU] Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Confers strong poly(A) specificity to the exosome (Probable).^[1] ^[2] [ECX2_ARCFU] Probably involved in the 3'->5' degradation of a variety of RNA species (Potential). [ECX1_ARCFU] Probably involved in the 3'->5' degradation of a variety of RNA species (Potential).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Exosomes emerge as central 3'-->5' RNA processing and degradation machineries in eukaryotes and archaea. We determined crystal structures of two 230 kDa nine subunit archaeal exosome isoforms. Both exosome isoforms contain a hexameric ring of RNase phosphorolytic (PH) domain subunits with a central chamber. Tungstate soaks identified three phosphorolytic active sites in this processing chamber. A trimer of Csl4 or Rrp4 subunits forms a multidomain macromolecular interaction surface on the RNase-PH domain ring with central S1 domains and peripheral KH and zinc-ribbon domains. Structural and mutational analyses suggest that the S1 domains and a subsequent neck in the RNase-PH domain ring form an RNA entry pore to the processing chamber that only allows access of unstructured RNA. This structural framework can mechanistically unify observed features of exosomes, including processive degradation of unstructured RNA, the requirement for regulatory factors to degrade structured RNA, and left-over tails in rRNA trimming.

Structural framework for the mechanism of archaeal exosomes in RNA processing.,Buttner K, Wenig K, Hopfner KP Mol Cell. 2005 Nov 11;20(3):461-71. PMID:16285927^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hartung S, Niederberger T, Hartung M, Tresch A, Hopfner KP. Quantitative analysis of processive RNA degradation by the archaeal RNA exosome. Nucleic Acids Res. 2010 Aug;38(15):5166-76. Epub 2010 Apr 14. PMID:20392821 doi:10.1093/nar/gkq238
↑ Buttner K, Wenig K, Hopfner KP. Structural framework for the mechanism of archaeal exosomes in RNA processing. Mol Cell. 2005 Nov 11;20(3):461-71. PMID:16285927 doi:10.1016/j.molcel.2005.10.018
↑ Buttner K, Wenig K, Hopfner KP. Structural framework for the mechanism of archaeal exosomes in RNA processing. Mol Cell. 2005 Nov 11;20(3):461-71. PMID:16285927 doi:10.1016/j.molcel.2005.10.018

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ba0"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2ba0]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BA0 FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ba1|2ba1]]</td></tr>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ba1|2ba1]]</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ba0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ba0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ba0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ba0 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ba0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ba0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ba0 RCSB], [http://www.ebi.ac.uk/pdbsum/2ba0 PDBsum]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/ECR1_ARCFU ECR1_ARCFU]] Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Confers strong poly(A) specificity to the exosome (Probable).<ref>PMID:20392821</ref> <ref>PMID:16285927</ref>  [[http://www.uniprot.org/uniprot/ECX2_ARCFU ECX2_ARCFU]] Probably involved in the 3'->5' degradation of a variety of RNA species (Potential). [[http://www.uniprot.org/uniprot/ECX1_ARCFU ECX1_ARCFU]] Probably involved in the 3'->5' degradation of a variety of RNA species (Potential).
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 29: / Line 31: @@
 </StructureSection>
 [[Category: Archaeoglobus fulgidus]]
-[[Category: Buttner, K.]]
+[[Category: Buttner, K]]
-[[Category: Hopfner, K P.]]
+[[Category: Hopfner, K P]]
-[[Category: Wenig, K.]]
+[[Category: Wenig, K]]
 [[Category: Archaeal]]
 [[Category: Exoribonuclease]]

2ba0

From Proteopedia

Revision as of 15:09, 24 December 2014

Archaeal exosome core

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox