1xmt
From Proteopedia
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| - | [[Image:1xmt.gif|left|200px]] | + | [[Image:1xmt.gif|left|200px]] |
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| - | '''X-ray structure of gene product from arabidopsis thaliana at1g77540''' | + | {{Structure |
| + | |PDB= 1xmt |SIZE=350|CAPTION= <scene name='initialview01'>1xmt</scene>, resolution 1.150Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=BR:BROMIDE ION'>BR</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= At1g77540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]) | ||
| + | }} | ||
| + | |||
| + | '''X-ray structure of gene product from arabidopsis thaliana at1g77540''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1XMT is a [ | + | 1XMT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XMT OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family., Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL, Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:[http:// | + | Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family., Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL, Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17128971 17128971] |
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: BR]] | [[Category: BR]] | ||
[[Category: at1g77540]] | [[Category: at1g77540]] | ||
| - | [[Category: center for eukaryotic structural | + | [[Category: center for eukaryotic structural genomic]] |
[[Category: cesg]] | [[Category: cesg]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
[[Category: putative acetyltransferase]] | [[Category: putative acetyltransferase]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:11:55 2008'' |
Revision as of 13:11, 20 March 2008
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| , resolution 1.150Å | |||||||
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| Ligands: | |||||||
| Gene: | At1g77540 (Arabidopsis thaliana) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-ray structure of gene product from arabidopsis thaliana at1g77540
Overview
We describe X-ray crystal and NMR solution structures of the protein coded for by Arabidopsis thaliana gene At1g77540.1 (At1g77540). The crystal structure was determined to 1.15 A with an R factor of 14.9% (Rfree = 17.0%) by multiple-wavelength anomalous diffraction using sodium bromide derivatized crystals. The ensemble of NMR conformers was determined with protein samples labeled with 15N and 13C + 15N. The X-ray structure and NMR ensemble were closely similar with rmsd 1.4 A for residues 8-93. At1g77540 was found to adopt a fold similar to that of GCN5-related N-acetyltransferases. Enzymatic activity assays established that At1g77540 possesses weak acetyltransferase activity against histones H3 and H4. Chemical shift perturbations observed in 15N-HSQC spectra upon the addition of CoA indicated that the cofactor binds and identified its binding site. The molecular details of this interaction were further elucidated by solving the X-ray structure of the At1g77540-CoA complex. This work establishes that the domain family COG2388 represents a novel class of acetyltransferase and provides insight into possible mechanistic roles of the conserved Cys76 and His41 residues of this family.
About this Structure
1XMT is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
Structure of Arabidopsis thaliana At1g77540 protein, a minimal acetyltransferase from the COG2388 family., Tyler RC, Bitto E, Berndsen CE, Bingman CA, Singh S, Lee MS, Wesenberg GE, Denu JM, Phillips GN Jr, Markley JL, Biochemistry. 2006 Dec 5;45(48):14325-36. PMID:17128971
Page seeded by OCA on Thu Mar 20 15:11:55 2008
Categories: Arabidopsis thaliana | Single protein | Allard, S T.M. | Bingman, C A. | Bitto, E. | CESG, Center for Eukaryotic Structural Genomics. | Jr., G N.Phillips. | Smith, D W. | Wesenberg, G E. | BR | At1g77540 | Center for eukaryotic structural genomic | Cesg | Protein structure initiative | Psi | Putative acetyltransferase | Structural genomic
