1xmq

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[[Image:1xmq.gif|left|200px]]<br /><applet load="1xmq" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1xmq.gif|left|200px]]
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caption="1xmq, resolution 3.0&Aring;" />
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'''Crystal Structure of t6A37-ASLLysUUU AAA-mRNA Bound to the Decoding Center'''<br />
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{{Structure
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|PDB= 1xmq |SIZE=350|CAPTION= <scene name='initialview01'>1xmq</scene>, resolution 3.0&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal Structure of t6A37-ASLLysUUU AAA-mRNA Bound to the Decoding Center'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1XMQ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=PAR:'>PAR</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XMQ OCA].
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1XMQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XMQ OCA].
==Reference==
==Reference==
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The role of modifications in codon discrimination by tRNA(Lys)UUU., Murphy FV 4th, Ramakrishnan V, Malkiewicz A, Agris PF, Nat Struct Mol Biol. 2004 Dec;11(12):1186-91. Epub 2004 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15558052 15558052]
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The role of modifications in codon discrimination by tRNA(Lys)UUU., Murphy FV 4th, Ramakrishnan V, Malkiewicz A, Agris PF, Nat Struct Mol Biol. 2004 Dec;11(12):1186-91. Epub 2004 Nov 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15558052 15558052]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: ribosome]]
[[Category: ribosome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:56:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:11:53 2008''

Revision as of 13:11, 20 March 2008

Image:1xmq.gif


PDB ID 1xmq

Drag the structure with the mouse to rotate
, resolution 3.0Å
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of t6A37-ASLLysUUU AAA-mRNA Bound to the Decoding Center


Overview

The natural modification of specific nucleosides in many tRNAs is essential during decoding of mRNA by the ribosome. For example, tRNA(Lys)(UUU) requires the modification N6-threonylcarbamoyladenosine at position 37 (t(6)A37), adjacent and 3' to the anticodon, to bind AAA in the A site of the ribosomal 30S subunit. Moreover, it can only bind both AAA and AAG lysine codons when doubly modified with t(6)A37 and either 5-methylaminomethyluridine or 2-thiouridine at the wobble position (mnm(5)U34 or s(2)U34). Here we report crystal structures of modified tRNA anticodon stem-loops bound to the 30S ribosomal subunit with lysine codons in the A site. These structures allow the rationalization of how modifications in the anticodon loop enable decoding of both lysine codons AAA and AAG.

About this Structure

1XMQ is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

The role of modifications in codon discrimination by tRNA(Lys)UUU., Murphy FV 4th, Ramakrishnan V, Malkiewicz A, Agris PF, Nat Struct Mol Biol. 2004 Dec;11(12):1186-91. Epub 2004 Nov 21. PMID:15558052

Page seeded by OCA on Thu Mar 20 15:11:53 2008

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