1xp8
From Proteopedia
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| - | [[Image:1xp8.jpg|left|200px]] | + | [[Image:1xp8.jpg|left|200px]] |
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| - | '''"Deinococcus radiodurans RecA in complex with ATP-gamma-S"''' | + | {{Structure |
| + | |PDB= 1xp8 |SIZE=350|CAPTION= <scene name='initialview01'>1xp8</scene>, resolution 2.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SAP:ADENOSINE-5'-DIPHOSPHATE MONOTHIOPHOSPHATE'>SAP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= recA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1299 Deinococcus radiodurans]) | ||
| + | }} | ||
| + | |||
| + | '''"Deinococcus radiodurans RecA in complex with ATP-gamma-S"''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1XP8 is a [ | + | 1XP8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XP8 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of RecA from Deinococcus radiodurans: insights into the structural basis of extreme radioresistance., Rajan R, Bell CE, J Mol Biol. 2004 Dec 3;344(4):951-63. PMID:[http:// | + | Crystal structure of RecA from Deinococcus radiodurans: insights into the structural basis of extreme radioresistance., Rajan R, Bell CE, J Mol Biol. 2004 Dec 3;344(4):951-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15544805 15544805] |
[[Category: Deinococcus radiodurans]] | [[Category: Deinococcus radiodurans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: recombination]] | [[Category: recombination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:12:45 2008'' |
Revision as of 13:12, 20 March 2008
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| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | recA (Deinococcus radiodurans) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
"Deinococcus radiodurans RecA in complex with ATP-gamma-S"
Overview
The resistance of Deinococcus radiodurans (Dr) to extreme doses of ionizing radiation depends on its highly efficient capacity to repair dsDNA breaks. Dr RecA, the key protein in the repair of dsDNA breaks by homologous recombination, promotes DNA strand-exchange by an unprecedented inverse pathway, in which the presynaptic filament is formed on dsDNA instead of ssDNA. In order to gain insight into the remarkable repair capacity of Dr and the novel mechanistic features of its RecA protein, we have determined its X-ray crystal structure in complex with ATPgammaS at 2.5A resolution. Like RecA from Escherichia coli, Dr RecA crystallizes as a helical filament that is closely related to its biologically relevant form, but with a more compressed pitch of 67 A. Although the overall fold of Dr RecA is similar to E.coli RecA, there is a large reorientation of the C-terminal domain, which in E.coli RecA has a site for binding dsDNA. Compared to E.coli RecA, the inner surface along the central axis of the Dr RecA filament has an increased positive electrostatic potential. Unique amino acid residues in Dr RecA cluster around a flexible beta-hairpin that has also been implicated in DNA binding.
About this Structure
1XP8 is a Single protein structure of sequence from Deinococcus radiodurans. Full crystallographic information is available from OCA.
Reference
Crystal structure of RecA from Deinococcus radiodurans: insights into the structural basis of extreme radioresistance., Rajan R, Bell CE, J Mol Biol. 2004 Dec 3;344(4):951-63. PMID:15544805
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