1xrx
From Proteopedia
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| - | [[Image:1xrx.gif|left|200px]] | + | [[Image:1xrx.gif|left|200px]] |
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| - | '''Crystal structure of a DNA-binding protein''' | + | {{Structure |
| + | |PDB= 1xrx |SIZE=350|CAPTION= <scene name='initialview01'>1xrx</scene>, resolution 2.15Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= seqA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of a DNA-binding protein''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1XRX is a [ | + | 1XRX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XRX OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a SeqA-N filament: implications for DNA replication and chromosome organization., Guarne A, Brendler T, Zhao Q, Ghirlando R, Austin S, Yang W, EMBO J. 2005 Apr 20;24(8):1502-11. Epub 2005 Mar 31. PMID:[http:// | + | Crystal structure of a SeqA-N filament: implications for DNA replication and chromosome organization., Guarne A, Brendler T, Zhao Q, Ghirlando R, Austin S, Yang W, EMBO J. 2005 Apr 20;24(8):1502-11. Epub 2005 Mar 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15933720 15933720] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein filament]] | [[Category: protein filament]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:13:47 2008'' |
Revision as of 13:13, 20 March 2008
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| , resolution 2.15Å | |||||||
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| Ligands: | |||||||
| Gene: | seqA (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a DNA-binding protein
Overview
Escherichia coli SeqA binds clusters of transiently hemimethylated GATC sequences and sequesters the origin of replication, oriC, from methylation and premature reinitiation. Besides oriC, SeqA binds and organizes newly synthesized DNA at replication forks. Binding to multiple GATC sites is crucial for the formation of stable SeqA-DNA complexes. Here we report the crystal structure of the oligomerization domain of SeqA (SeqA-N). The structural unit of SeqA-N is a dimer, which oligomerizes to form a filament. Mutations that disrupt filament formation lead to asynchronous DNA replication, but the resulting SeqA dimer can still bind two GATC sites separated from 5 to 34 base pairs. Truncation of the linker between the oligomerization and DNA-binding domains restricts SeqA to bind two GATC sites separated by one or two full turns. We propose a model of a SeqA filament interacting with multiple GATC sites that accounts for both origin sequestration and chromosome organization.
About this Structure
1XRX is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of a SeqA-N filament: implications for DNA replication and chromosome organization., Guarne A, Brendler T, Zhao Q, Ghirlando R, Austin S, Yang W, EMBO J. 2005 Apr 20;24(8):1502-11. Epub 2005 Mar 31. PMID:15933720
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