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1xt3

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[[Image:1xt3.gif|left|200px]]<br /><applet load="1xt3" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1xt3.gif|left|200px]]
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caption="1xt3, resolution 2.4&Aring;" />
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'''Structure Basis of Venom Citrate-Dependent Heparin Sulfate-Mediated Cell Surface Retention of Cobra Cardiotoxin A3'''<br />
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{{Structure
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|PDB= 1xt3 |SIZE=350|CAPTION= <scene name='initialview01'>1xt3</scene>, resolution 2.4&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CIT:CITRIC ACID'>CIT</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Structure Basis of Venom Citrate-Dependent Heparin Sulfate-Mediated Cell Surface Retention of Cobra Cardiotoxin A3'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1XT3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra] with <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT3 OCA].
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1XT3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT3 OCA].
==Reference==
==Reference==
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Structural basis of citrate-dependent and heparan sulfate-mediated cell surface retention of cobra cardiotoxin A3., Lee SC, Guan HH, Wang CH, Huang WN, Tjong SC, Chen CJ, Wu WG, J Biol Chem. 2005 Mar 11;280(10):9567-77. Epub 2004 Dec 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15590643 15590643]
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Structural basis of citrate-dependent and heparan sulfate-mediated cell surface retention of cobra cardiotoxin A3., Lee SC, Guan HH, Wang CH, Huang WN, Tjong SC, Chen CJ, Wu WG, J Biol Chem. 2005 Mar 11;280(10):9567-77. Epub 2004 Dec 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15590643 15590643]
[[Category: Naja atra]]
[[Category: Naja atra]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: heparin]]
[[Category: heparin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:58:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:14:14 2008''

Revision as of 13:14, 20 March 2008

Image:1xt3.gif


PDB ID 1xt3

Drag the structure with the mouse to rotate
, resolution 2.4Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



Structure Basis of Venom Citrate-Dependent Heparin Sulfate-Mediated Cell Surface Retention of Cobra Cardiotoxin A3


Overview

Anionic citrate is a major component of venom, but the role of venom citrate in toxicity other than its inhibitory effect on the cation-dependent action of venom toxins is poorly understood. By immobilizing Chinese hamster ovary cells in microcapillary tubes and heparin on sensor chips, we demonstrated that heparan sulfate-mediated cell retention of the major cardiotoxin (CTX) from the Taiwan cobra, CTX A3, near membrane surfaces is citrate-dependent. X-ray determination of a CTX A3-heparin hexasaccharide complex structure at 2.4 A resolution revealed a molecular mechanism for toxin retention in which heparin-induced conformational changes of CTX A3 lead to citrate-mediated dimerization. A citrate ion bound to Lys-23 and Lys-31 near the tip of loop II stabilizes hydrophobic contact of the CTX A3 homodimer at the functionally important loop I and II regions. Additionally, the heparin hexasaccharide interacts with five CTX A3 molecules in the crystal structure, providing another mechanism whereby the toxin establishes a complex network of interactions that result in a strong interaction with cell surfaces presenting heparan sulfate. Our results suggest a novel role for venom citrate in biological activity and reveal a structural model that explains cell retention of cobra CTX A3 through heparan sulfate-CTX interactions.

About this Structure

1XT3 is a Single protein structure of sequence from Naja atra. Full crystallographic information is available from OCA.

Reference

Structural basis of citrate-dependent and heparan sulfate-mediated cell surface retention of cobra cardiotoxin A3., Lee SC, Guan HH, Wang CH, Huang WN, Tjong SC, Chen CJ, Wu WG, J Biol Chem. 2005 Mar 11;280(10):9567-77. Epub 2004 Dec 6. PMID:15590643

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