4boc
From Proteopedia
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| - | + | ==Structure of mitochondrial RNA polymerase elongation complex== | |
| - | + | <StructureSection load='4boc' size='340' side='right' caption='[[4boc]], [[Resolution|resolution]] 2.65Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4boc]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BOC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BOC FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4boc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4boc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4boc RCSB], [http://www.ebi.ac.uk/pdbsum/4boc PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/RPOM_HUMAN RPOM_HUMAN]] DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.<ref>PMID:21278163</ref> | [[http://www.uniprot.org/uniprot/RPOM_HUMAN RPOM_HUMAN]] DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.<ref>PMID:21278163</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Here we report the crystal structure of the human mitochondrial RNA polymerase (mtRNAP) transcription elongation complex, determined at 2.65-A resolution. The structure reveals a 9-bp hybrid formed between the DNA template and the RNA transcript and one turn of DNA both upstream and downstream of the hybrid. Comparisons with the distantly related RNA polymerase (RNAP) from bacteriophage T7 indicates conserved mechanisms for substrate binding and nucleotide incorporation but also strong mechanistic differences. Whereas T7 RNAP refolds during the transition from initiation to elongation, mtRNAP adopts an intermediary conformation that is capable of elongation without refolding. The intercalating hairpin that melts DNA during T7 RNAP initiation separates RNA from DNA during mtRNAP elongation. Newly synthesized RNA exits toward the pentatricopeptide repeat (PPR) domain, a unique feature of mtRNAP with conserved RNA-recognition motifs. | ||
| + | |||
| + | Structure of human mitochondrial RNA polymerase elongation complex.,Schwinghammer K, Cheung AC, Morozov YI, Agaronyan K, Temiakov D, Cramer P Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2683. PMID:24096365<ref>PMID:24096365</ref> | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[RNA polymerase|RNA polymerase]] | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Human]] | [[Category: Human]] | ||
| - | [[Category: Agaronyan, K | + | [[Category: Agaronyan, K]] |
| - | [[Category: Cheung, A | + | [[Category: Cheung, A]] |
| - | [[Category: Cramer, P | + | [[Category: Cramer, P]] |
| - | [[Category: Morozov, Y | + | [[Category: Morozov, Y]] |
| - | [[Category: Schwinghammer, K | + | [[Category: Schwinghammer, K]] |
| - | [[Category: Temiakov, D | + | [[Category: Temiakov, D]] |
[[Category: Dna-rna hybrid]] | [[Category: Dna-rna hybrid]] | ||
[[Category: Mitochondria]] | [[Category: Mitochondria]] | ||
Revision as of 16:03, 24 December 2014
Structure of mitochondrial RNA polymerase elongation complex
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