1xt9
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1xt9.gif|left|200px]] | + | [[Image:1xt9.gif|left|200px]] |
| - | + | ||
| - | '''Crystal Structure of Den1 in complex with Nedd8''' | + | {{Structure |
| + | |PDB= 1xt9 |SIZE=350|CAPTION= <scene name='initialview01'>1xt9</scene>, resolution 2.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= SENP8, FKSG8, PRSC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), NEDD8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Den1 in complex with Nedd8''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1XT9 is a [ | + | 1XT9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XT9 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1., Reverter D, Wu K, Erdene TG, Pan ZQ, Wilkinson KD, Lima CD, J Mol Biol. 2005 Jan 7;345(1):141-51. PMID:[http:// | + | Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1., Reverter D, Wu K, Erdene TG, Pan ZQ, Wilkinson KD, Lima CD, J Mol Biol. 2005 Jan 7;345(1):141-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15567417 15567417] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 22: | Line 31: | ||
[[Category: ubiquitin-like]] | [[Category: ubiquitin-like]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:14:21 2008'' |
Revision as of 13:14, 20 March 2008
| |||||||
| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | SENP8, FKSG8, PRSC2 (Homo sapiens), NEDD8 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Den1 in complex with Nedd8
Overview
The Nedd8 conjugation pathway is conserved from yeast to humans and is essential in many organisms. Nedd8 is conjugated to cullin proteins in a process that alters SCF E3 ubiquitin ligase activity, and it is presumed that Nedd8 deconjugation would reverse these effects. We now report the X-ray structures of the human Nedd8-specific protease, Den1, in a complex with the inhibitor Nedd8 aldehyde, thus revealing a model for the tetrahedral transition state intermediate generated during proteolysis. Although Den1 is closely related to the SUMO-specific protease family (Ulp/Senp family), structural analysis of the interface suggests determinants involved in Nedd8 selectivity by Den1 over other ubiquitin-like family members and suggests how the Ulp/Senp architecture has been modified to interact with different ubiquitin-like modifiers.
About this Structure
1XT9 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1., Reverter D, Wu K, Erdene TG, Pan ZQ, Wilkinson KD, Lima CD, J Mol Biol. 2005 Jan 7;345(1):141-51. PMID:15567417
Page seeded by OCA on Thu Mar 20 15:14:21 2008
